The Vegetative Transcriptome of Arabidopsis Thaliana

拟南芥植物转录组

• 批准号: 0725968
• 负责人: R. Poethig
• 金额: $ 68.06万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-15 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0725968&HistoricalAwards=false
• 关键词: Vegetative; Transcriptome; Arabidopsis; Thaliana

The morphology, physiology, and biochemical composition of leaves produced at different times in the development of a plant changes in a predictable fashion. Particularly dramatic changes in leaf morphology and physiology occur when a plant is induced to flower. The molecular basis for this phenomenon will be studied by performing a genome-wide analysis of gene expression in leaves at different positions on the shoot of flowering and non-flowering plants of Arabidopsis thaliana. Genes whose expression changes in accordance with the transition between juvenile and adult stages of shoot development are of particular interest, and will be identified by comparing gene expression patterns in normal plants with plants that have been "juvenilized" by the constitutive expression of miR156. mRNA abundance will be determined using the Affymetrix ATH1 microarray, whereas small RNA abundance will be determined by high-throughput sequencing-by-synthesis technology. These data will be accessible via a project-specific page at http://mpss.udel.edu/at/ and will be deposited in the Gene Expression Omnibus (GEO) at NCBI for long-term storage and dissemination.Broader Impacts: Leaves are the primary photosynthetic organ of a plant and are therefore the most important source of renewable energy on earth. The information obtained in this study will extend our understanding of plant gene regulatory networks and make it possible to rationally engineer leaf morphology and physiology to maximize plant performance. Importantly the project will also provide for the training of the next generation of plant scientists in cutting edge functional genomic technologies.

植物发育过程中不同时期产生的叶子的形态、生理和生化成分以可预测的方式发生变化。当植物被诱导开花时，叶子形态和生理学会发生特别显着的变化。将通过对拟南芥开花和不开花植物芽上不同位置的叶子中的基因表达进行全基因组分析来研究这种现象的分子基础。其表达随着枝条发育的幼年阶段和成年阶段之间的转变而变化的基因特别令人感兴趣，并且将通过比较正常植物和通过miR156的组成型表达而“幼化”的植物中的基因表达模式来鉴定。 mRNA 丰度将使用 Affymetrix ATH1 微阵列测定，而小 RNA 丰度将通过高通量合成测序技术测定。这些数据可通过 http://mpss.udel.edu/at/ 上的项目特定页面访问，并将存储在 NCBI 的基因表达综合库 (GEO) 中进行长期存储和传播。 更广泛的影响：叶子是植物的主要光合作用器官，因此是地球上最重要的可再生能源。本研究中获得的信息将扩展我们对植物基因调控网络的理解，并使合理设计叶片形态和生理学以最大限度地提高植物性能成为可能。 重要的是，该项目还将为下一代植物科学家提供尖端功能基因组技术的培训。