Genetic Regulation of Phase Change in Maize

玉米相变的遗传调控

• 批准号: 9205739
• 负责人: R. Poethig
• 金额: $ 32.25万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9205739&HistoricalAwards=false
• 关键词: Genetic; Regulation; Phase; Change; Maize

During its growth the maize shoot progresses through distinct juvenile and adult phases of vegetative growth and then enters a reproductive phase. Previous work has suggested that these three phases are regulated by independent, but interacting developmental programs. The extent of coordination between these programs will be tested by studying the effect of "early flowering" genes on phase-specific vegetative traits, and by examining the interaction between these genes and several mutations that cause the constitutive expression of a juvenile vegetative program (Tp1, Tp2 and Tp3). To obtain a more complete picture of the genetic regulation of phase change, several recently identified mutations that affect the expression of phase specific vegetative traits will be characterized at a cellular and molecular level. Other genes that regulate phase change will be identified by screening for suppressors of Tp2, and by screening for mutations that cause the precocious expression of adult vegetative traits. Finally, g115, a gene that regulates several juvenile traits, will be cloned by transposon-tagging.

在其生长过程中，玉米芽经历不同的幼年和成年营养生长阶段，然后进入生殖阶段。 先前的研究表明，这三个阶段是由独立但相互作用的发育程序调节的。 这些程序之间的协调程度将通过研究“早期开花”基因对特定阶段营养性状的影响，并通过检查这些基因与导致幼年营养程序（Tp1）组成型表达的几种突变之间的相互作用来测试。 、Tp2 和 Tp3)。 为了更全面地了解相变的遗传调控，最近发现的几个影响相特异性营养性状表达的突变将在细胞和分子水平上进行表征。 其他调节相变的基因将通过筛选 Tp2 抑制因子和筛选导致成体营养性状早熟表达的突变来鉴定。 最后，g115（一种调节多种幼体性状的基因）将通过转座子标记进行克隆。