The Function of HASTY in Arabidopsis

HASTY在拟南芥中的功能

• 批准号: 0346050
• 负责人: R. Poethig
• 金额: --
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2007-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0346050&HistoricalAwards=false
• 关键词: Function; HASTY; Arabidopsis

The transport of molecules across the nuclear membrane is primarily regulated by members of the importin beta family of proteins. Although the function of these proteins is well characterized in yeast and mammals, very little is known about their function in plants. The experiments described in this project are designed to reveal the function of HASTY, the Arabidopsis orthologue of Exportin5. Exportin5 has been implicated in the nuclear export of miRNAs and tRNAs in mammals, and the mutant phenotype of hasty suggests that it may have a similar function in plants. This hypothesis will be tested by examining the effect of hasty mutations on the sub-cellular distribution of miRNAs and tRNAs, and by identifying the molecules with which the HASTY protein physically interacts in vivo and in vitro. Screens for mutations that suppress the phenotype of hst-3 have been conducted in order to identify genes that are functionally related to HST. Positional cloning will be used to identify the gene affected by one such mutation, soh, and its function will be characterized. At present, HASTY is the only orthologue of Exportin5 in higher organisms for which mutant alleles exist. These mutations provide a unique opportunity to study the function of this important protein, and may provide new insights into the biogenesis and function of miRNAs as well.

分子在整个核膜中的运输主要由蛋白质β家族的成员调节。尽管这些蛋白质的功能在酵母和哺乳动物中表现得很好，但对它们在植物中的功能知之甚少。该项目中描述的实验旨在揭示出口的拟南芥直系同源物的Hasty的功能。 Exportin5与哺乳动物中的miRNA和TRNA的核出口有关，仓促的突变表型表明它在植物中可能具有相似的功能。该假设将通过检查仓促突变对miRNA和TRNA的亚细胞分布的影响，并通过鉴定仓促蛋白在体内和体外与仓促蛋白质物理相互作用的分子进行检验。为了鉴定与HST功能相关的基因，已经进行了抑制HST-3表型突变的筛选。位置克隆将用于识别受一种突变，SOH影响的基因，其功能将被表征。目前，Hasty是较高生物体中存在突变等位基因的唯一出口源。 这些突变为研究这种重要蛋白质的功能提供了独特的机会，并可能还可以为miRNA的生物发生和功能提供新的见解。