Identification of cell fate specification mechanisms during early embryogenesis in Arabidopsis

拟南芥早期胚胎发生过程中细胞命运规范机制的鉴定

• 批准号: 22KF0023
• 负责人: 植田 美那子
• 金额: $ 1.47万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0023/
• 关键词: Arabidopsis thaliana; embryogenesis; transcriptome; cellular dynamics

This study aims to identify the key genes involved during embryogenesis in Arabidopsis thaliana (Arabidopsis). Zygotic embryogenesis is a fundamental developmental process in which a single-celled zygote goes through a series of cell divisions, patterning and cell type specifications to form a complex embryo during eukaryotic sexual reproduction. In the flowering plant Arabidopsis, the zygotes divide with a stereotypic pattern, making them an ideal system to observe embryo morphology and to trace cell lineage. While decades of genetic analyses have identified a number of genes that play vital roles in embryogenesis, the genome-wide analyses were not feasible until recently due to technical difficulties. We utilized published embryonic transcriptome datasets to search for key regulatory genes of embryogenesis as well as to resolve the underlying regulatory networks. With bioinformatics approaches, we selected a number of candidate genes for further validations and analyses. Identifying the key factors involved in embryonic cell divisions and specifications will greatly improve our knowledge of flowering plant embryogenesis mechanisms, which is important for understanding the fundamental biological process enabling multicellular eukaryotic lives.

本研究旨在鉴定拟南芥（Arabidopsis）胚胎发生过程中涉及的关键基因。合子胚胎发生是一个基本的发育过程，其中单细胞受精卵在真核有性生殖过程中经历一系列细胞分裂、模式化和细胞类型规范，形成复杂的胚胎。在开花植物拟南芥中，受精卵以固定模式分裂，使其成为观察胚胎形态和追踪细胞谱系的理想系统。虽然数十年的遗传分析已经确定了许多在胚胎发生中发挥重要作用的基因，但由于技术困难，全基因组分析直到最近才可行。我们利用已发表的胚胎转录组数据集来寻找胚胎发生的关键调控基因并解析潜在的调控网络。通过生物信息学方法，我们选择了一些候选基因进行进一步验证和分析。识别胚胎细胞分裂和规格中涉及的关键因素将极大地提高我们对开花植物胚胎发生机制的了解，这对于理解实现多细胞真核生命的基本生物过程非常重要。

项目成果

Wageningen University(オランダ)

瓦赫宁根大学（荷兰）