Collaborative Research: Biotransformations Near and Above 100C: Hyperthermophilic Microorganisms and Enzymes for Bioenergy Conversion

合作研究：100C 附近及以上的生物转化：用于生物能源转化的超嗜热微生物和酶

• 批准号: 0617235
• 负责人: Michael Adams
• 金额: $ 42万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0617235&HistoricalAwards=false
• 关键词: Collaborative; Research; Biotransformations; Near; Above

0617235AdamsIdentification of microorganisms and biocatalysts for bioenergy conversion processes remains a key objective in the development of alternative fuels. Recently available genomics-based approaches offer unprecedented access to novel enzymes and pathways for biomass conversions. The objective of this research is to identify novel biocatalytic and metabolic strategies for bioenergy conversion by using functional and structural genomics-based methods, in conjunction with microbial and biochemical approaches. The intellectual merit of the proposed research is the strategic use of classical and modern approaches to study extremophile biology and biotechnology as this relates to bioenergy conversion. The specific objectives of the project are: (1) to use whole genome transcriptional response analyses of four hyperthermophilic hydrogen-producing anaerobes to identify novel biocatalyst targets for processing biomass to substrates that can be readily converted to biofuels; (2) to produce key biocatalysts for biochemical characterization and to assess their roles in bioenergy production; (3) to compare hydrogen production rates and yields for the four hyperthemophilic anaerobes. (4) to evaluate bioprocess strategies for biohydrogen production at elevated temperatures by hyperthermophile cultures. The broader impact of this work relates to the interdisciplinary training of graduate students in the use of functional genomics for studying biocatalysis and biotransformations in extremophilic microorganisms. The two participating laboratories will continue their longstanding efforts to involve undergraduate students and students from underrepresented minority groups in the research.

0617235ADAMSSIDICY鉴定微生物和生物能催化剂用于生物能量转化过程仍然是替代燃料开发的关键目标。最近可用的基于基因组学的方法为生物量转化的新酶和途径提供了前所未有的访问。这项研究的目的是通过使用基于功能和结构基因组学的方法来确定生物能源转化的新型生物催化和代谢策略，并结合微生物和生化方法。拟议的研究的智力优点是将经典和现代方法的战略用途用于研究极端生物学和生物技术，这与生物能量转化有关。该项目的具体目标是：（1）使用四个高嗜热氢的厌氧菌的整个基因组转录反应分析，以鉴定新的生物催化剂靶标，以将生物量处理到可以很容易地转化为生物燃料的底物的生物量； （2）生产关键的生物催化剂，以进行生化特征并评估其在生物能源生产中的作用； （3）比较四种过度嗜好的厌氧菌的氢产量和产量。 （4）评估高疗法培​​养物在升高温度下生物氢生产的生物过程策略。这项工作的更广泛的影响与研究生在使用功能基因组学用于研究极端微生物的生物催化和生物转化方面的跨学科培训有关。这两个参与的实验室将继续他们的长期努力，以使本科生和来自代表性不足的少数群体的学生参与研究。