Signaling to Cell Morphogenesis in Arabidopsis Leaves

拟南芥叶细胞形态发生的信号传导

• 批准号: 0417255
• 负责人: Zhenbiao Yang
• 金额: $ 39万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0417255&HistoricalAwards=false
• 关键词: Signaling; Cell; Morphogenesis; Arabidopsis; Leaves

The long-term goal of this project is to understand how plant cells achieve various shapes. Cell shape formation is important for the development and the morphogenesis of plants, but the molecular mechanism underlying this fundamental process is poorly understood. This project focuses on a network of regulatory proteins in the control of interlocking jigsaw puzzle appearance of Arabidopsis leaf epidermal cells. A central player in this network is a plant-specific guanine nucleotide binding protein known as ROP. The formation of jigsaw puzzle appearance requires the coordination of localized cellular outgrowth in the lobe-forming region of the cell with localized constraint of outgrowth in the other regions. ROP promotes lobing by activating the RIC4 protein to assemble the actin cytoskeleton required for localized cell outgrowth, as well as by inactivating the RIC1 protein that organizes the microtubule cytoskeleton for constraining cellular outgrowth. The RIC1 pathway feedback represses ROP activation to assure the formation of indentations. In this proposed work, genetic, molecular, cell biological, and proteomic approaches will be used to elucidate the mechanism by which ROP activity is regulated as well as the mechanism underpinning the RIC1 regulation of microtubules. This research aim is expected to provide new insights into the mechanism for cell shape formation in plants and to identify new genes that control cell morphogenesis and plant development. These genes may prove to be useful for genetically manipulating plant forms that are beneficial to agricultural and horticultural practices. This project will also integrate research with training of undergraduate and graduate students and participation of underrepresented undergraduate students through outreach programs such as Minority Student Summer Research Internships and REU Programs.

该项目的长期目标是了解植物细胞如何实现各种形状。细胞形状的形成对于植物的发育和形态发生很重要，但是这种基本过程的分子机制知之甚少。 该项目着重于调节蛋白网络，以控制拟南芥叶表皮细胞的互锁拼图拼图。 该网络中的核心参与者是一种植物特异性鸟嘌呤核苷酸结合蛋白，称为ROP。 拼图拼图的形成需要在细胞的叶片形成区域中协调局部细胞出生，并在其他区域中具有局部生长约束。 ROP通过激活RIC4蛋白来促进LOP，以组装局部细胞出生所需的肌动蛋白细胞骨架，以及通过灭活微管细胞骨架来限制细胞生长的RIC1蛋白。 RIC1途径反馈抑制ROP激活以确保压痕的形​​成。 在这项提出的工作中，将使用遗传，分子，细胞生物学和蛋白质组学方法来阐明调节ROP活性的机制以及基于微管的RIC1调节的机制。 这项研究的目的预计将提供有关植物细胞形成机制的新见解，并确定控制细胞形态发生和植物发育的新基因。 这些基因可能被证明对有益于农业和园艺实践的遗传操纵植物形式有用。 该项目还将通过培训本科生和研究生的培训以及通过少数学生夏季研究实习和REU计划等宣传计划的培训以及代表性不足的本科生的参与。