Arabidopsis 2010: Chemical Genomics of Networks Controlling Vesicular Trafficking in Plant Development

拟南芥 2010：植物发育中控制囊泡运输的网络化学基因组学

• 批准号: 0520325
• 负责人: Zhenbiao Yang
• 金额: $ 100万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0520325&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Chemical; Genomics; Networks

SCIENTIFIC MERIT: This project is to demonstrate that integration of an innovative and state-of-the-art chemical genomics approach with genomic tools is useful for elucidating networks of genes that control vesicular trafficking critical for Arabidopsis growth and development. Gene networks that are specific to plants, e.g., those that control plant cell division or polar transport of the plant hormone auxin, will be of particular interest in this pilot project. The chemical genomics approach is proposed because these networks have proven intractable to traditional genetic/genomics approaches alone. Chemical genomics is an emerging field, in which high-throughput technologies are employed to identify bioactive small molecules, which in turn are used to interrogate a complex biological process or its underlying network of genes. The following scientific goals will be achieved: 1) Discover a battery of small molecules that perturb the control of cell division that requires vesicular trafficking, 2) Initiate assigning the functions to a network of genes controlling cell division in Arabidopsis by integrating the chemical genomics approach with the genomics tools developed through previous 2010 projects (e.g., SIGNAL), and 3) Develop a web-based mechanism by which tools, materials, chemicals, and knowledge generated in this project can be easily disseminated to the public (http://bioinfo.ucr.edu/projects/PlantChemBase/search.php; usename: visitor, password: chemical). The long-term goal of this project is to complement and enhance the existing functional genomics/genomics tools for the discovery of the functions of genes in Arabidopsis.BROADER IMPACT: This project will have a broad impact on plant biology by proving the usefulness of chemical genomics in the research on gene function and regulatory networks. Tools and materials, developed in this project, e.g., high-throughput imaging-based chemical screen routines and novel genetics and molecular tools including mutants, transgenic lines, protein expression vectors, and sub-cellular markers will be made available to the public. An integrated database for compound mining, retrieval of screening data, phenotypes and downloadable open-source tools for compound analysis will be created and published through a centralized suite of Web services. This project will integrate training of young scientists with the goal of providing them with a broad integrated experience within interdisciplinary chemical genomics research. The future scientists will be well trained and prepared for independent academic careers. Focused outreach efforts will be undertaken to stimulate interest in this leading edge of science among women and under-represented minority students. This training will produce a new generation of diverse scientists who can form complementary teams required for 21st century research careers.

科学优点：该项目是为了证明，将创新和最先进的化学基因组学方法与基因组工具的整合在一起，可用于阐明控制囊泡运输对拟南芥增长和发展至关重要的基因网络。 特有植物的基因网络，例如控制植物细胞分裂或植物激素生长素的极性转运的基因网络，将在该试验项目中特别感兴趣。 提出了化学基因组学方法，因为这些网络仅对传统的遗传/基因组学方法进行了证明。 化学基因组学是一个新兴领域，其中采用高通量技术来鉴定生物活性小分子，而生物活性小分子又用于询问复杂的生物学过程或其基础基因网络。 将实现以下科学目标：1）发现需要囊泡贩运的细胞分裂控制的一组小分子，2）通过整合化学基因组学方法，将功能分配给控制拟南芥中细胞分裂的基因网络借助通过先前的2010年项目（例如信号）开发的基因组学工具，并开发了一种基于网络的机制，通过该机制，该项目中产生的工具，材料，化学物质和知识可以轻松地传播给公众（http：// http：// bioinfo.ucr.edu/projects/plantchembase/search.php; 该项目的长期目标是补充和增强现有的功能基因组/基因组学工具，以发现基因在拟南芥中的功能。Broader的影响：该项目将通过证明化学的实用性对植物生物学产生广泛的影响基因组基因组学和调节网络的基因组学。该项目中开发的工具和材料，例如，基于高通量成像的化学筛选程序以及新型遗传学和分子工具，包括突变体，转基因线，蛋白质表达载体和亚细胞标记物，将提供给公众。用于复合挖掘的集成数据库，筛选数据的检索，表型和可下载的开源工具，用于复合分析，并通过集中的Web服务套件来创建和发布。该项目将融合对年轻科学家的培训，以便为他们提供跨学科化学基因组学研究中的广泛综合经验。 未来的科学家将经过良好的培训，并为独立的学术职业做好准备。 将采取重点的外展工作，以激发妇女和代表性不足的少数民族学生对科学领先的兴趣。这项培训将产生新一代的不同科学家，他们可以组成21世纪研究​​职业所需的互补团队。