Plant Protein Farnesyltransferase: Role in the Cell Cycle Control

植物蛋白法尼基转移酶：在细胞周期控制中的作用

• 批准号: 9604681
• 负责人: Zhenbiao Yang
• 金额: $ 5万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-15 至 1998-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604681&HistoricalAwards=false
• 关键词: Plant; Protein; Farnesyltransferase; Role; Cell

9604681 Yang Protein farnesyltransferase (FTase)-mediated attachment of a lipid (i.e., farnesyl) moiety to proteins facilitates translocation to the plasma membrane and thus activation of signaling proteins such as p21ras. As a result, FTase plays an important role in many signaling pathways. Recent studies suggest that FTase plays a critical role in abscisic acid signaling and cell cycle control in plants. However, farnesylated proteins involved in these plant signaling events have not been identified. In our preliminary studies, an Arabidopsis Ras-like small GTP-binding protein (RLP1) was shown to be farnesylated in vitro, and its full-length cDNA was sequenced. This represents the first plant signaling protein known to be farnesylated and provides a strong candidate for a farnesylated protein in ABA signaling or cell cycle control. In this revised objective, we will investigate the function of this G-protein in plants. Two specific experiments will be conducted. 1) RLP1 will be fused to the jellyfish green fluorescence protein and expressed in tobacco BY-2 cells to demonstrate in vivo farnesylation of RLP1 and to determine its subcellular localization. 2) Dominant negative and constitutively active mutant forms of RLP1 will be created by site-directed mutagenesis and expressed in BY-2 cells to directly assess its role in plant signaling pathways such as ABA or mitogenic signaling. This work will provide significant new insight into the role of farnesylation and farnesylated proteins in signal transduction. This project will examine the a signal processes that are involved in regulating cell division in plant cells. Understanding how plant cell division is regulated is an important fundamental process with implications in biotechnology and agriculture. Dr. Yang has discovered that one protein that may be involved in signaling during cell division is modified with the attachment of a lipid. The attachment and removal of the lipid may be one of the essential steps in the signali ng process. This project will provide detailed information on the correlation between lipid modification of the protein and the regulation of plant cell division.

9604681杨蛋白Farnesylsylansferase（FTase）介导的脂质（即Farnesyl）部分对蛋白质的附着促进了易位到质膜，从而激活信号蛋白（例如P21RAS）。 结果，FTase在许多信号通路中起着重要作用。 最近的研究表明，FTase在植物中的脱离酸信号传导和细胞周期控制中起着至关重要的作用。 但是，尚未确定参与这些植物信号事件的法尼基化蛋白。 在我们的初步研究中，拟南芥Ras样小的GTP结合蛋白（RLP1）在体外被证明是法尼化的，并且对其全长cDNA进行了测序。 这代表了已知的Farneylation的第一个植物信号蛋白，并为ABA信号传导或细胞周期控制中的Farnesylated蛋白提供了强有力的候选者。 在这个修订的目标中，我们将研究该G蛋白在植物中的功能。 将进行两个具体的实验。 1）RLP1将融合到水母绿色荧光蛋白上，并在烟草By-2细胞中表达，以证明RLP1的体内法尼化并确定其亚细胞定位。 2）RLP1的主要负极和组成性活性突变形式将由定点诱变创建，并在By-2细胞中表达，以直接评估其在ABA或有丝分裂信号等植物信号途径中的作用。 这项工作将为Farnesylation和Farnesylation蛋白在信号转导中的作用提供重大的新见解。 该项目将检查与调节植物细胞中细胞分裂有关的A信号过程。了解如何调节植物细胞分裂是一个重要的基本过程，对生物技术和农业有影响。杨博士发现，通过脂质的附着，可以修饰一种可能参与信号传导的蛋白质。脂质的附着和去除可能是信号过程中的重要步骤之一。该项目将提供有关蛋白质脂质修饰与植物细胞分裂调节之间相关性的详细信息。