Infrared Matrix Assisted Laser Desorption Ionization Mass Spectrometry

红外基质辅助激光解吸电离质谱法

• 批准号: 0415360
• 负责人: Kermit Murray
• 金额: $ 35.85万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0415360&HistoricalAwards=false
• 关键词: Infrared; Matrix; Assisted; Laser; Desorption

Professor Kermit Murray of Louisiana State University is supported by the Analytical and Surface Chemistry Program to further the development and application of infrared matrix-assisted laser desorption ionization mass spectrometry (IR-MALDI). IR-MALDI is not as widely accepted a method as MALDI using ultraviolet (UV) radiation, but offers some advantages including the ability to produce ions with less internal energy, the ability to produce ions without the use of a matrix, and the ability to characterize low molecular weight analytes without low mass interferences. Fundamental studies of IR laser desorption, two-pulse IR/UV MALDI, and particle ablation are being conducted as is the development of "direct from gel" IR laser desorption ionization strategies for peptide characterization. The PI uses a novel web-based Virtual Lab Tour to introduce college students to mass spectrometry and to introduce the public to what scientific researchers do on a daily basis.Mass spectrometry in various forms has proven essential to the high-throughput highly accurate analysis of biological molecules. Given the high impact the UV-MALDI-MS technique has as a sensitive analytical technique (in particular in proteomics and increasingly in biomedical analysis), prospects for IR-MALDI are promising. This project outlines improvement of instrumentation and techniques in the area of mass spectrometry of biological molecules, as well as development of new approaches and applications that should expand the role of MALDI applications such as biomedical research, clinical measurements, and synthetic polymers.

路易斯安那州立大学的Kermit Murray教授得到了分析和表面化学计划的支持，以进一步发展和应用红外矩阵辅助激光解吸电离电离质谱法（IR-Maldi）。 使用紫外线（UV）辐射，IR-Maldi并不像MALDI那样广泛接受，但具有一些优势，包括能够生产较少内部能量的离子，在不使用矩阵的情况下产生离子的能力以及表征低分子量分析的能力，而没有低质量干扰。对IR激光解吸，两脉冲IR/UV Maldi和颗粒消融的基本研究正在进行肽表征的“直接从凝胶” IR激光解吸策略中的开发。 PI使用新型的基于Web的虚拟实验室巡回演出，向大学生介绍质谱法，并向公众介绍科学研究人员每天所做的事情。各种形式的质谱学已证明对高通量的高通量对生物分子的高度准确分析至关重要。 鉴于UV-Maldi-MS技术具有很高的影响，作为一种敏感的分析技术（尤其是在蛋白质组学中，在生物医学分析中越来越多），IR-Maldi的前景很有希望。 该项目概述了生物分子质谱领域的仪器和技术的改进以及应扩大MALDI应用作用的新方法和应用的开发，例如生物医学研究，临床测量和合成聚合物。