MRI: Acquisition of a MALDI Tandem Mass Spectrometer (MALDI MS/MS) for Imaging, Biological Research and Chemical Materials Characterization

MRI：购买 MALDI 串联质谱仪 (MALDI MS/MS) 用于成像、生物研究和化学材料表征

• 批准号: 2215823
• 负责人: Kermit Murray
• 金额: $ 47.4万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2215823&HistoricalAwards=false
• 关键词: MRI; Acquisition; MALDI; Tandem; Mass

This award is jointly funded by the Major Research Instrumentation Program, the Chemistry Research Instrumentation Program, and the Established Program to Stimulate Competitive Research (EPSCoR). Professor Kermit Murray from Louisiana State University, on behalf of from 11 investigators in 4 departments across the university, is acquiring a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF-MS) equipped with a photodiode array. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide range of samples. The acquisition strengthens the research infrastructure at the university and within the regional. The instrument broadens participation by giving hands-on access to a diverse student population. The instrument is also used in outreach activities. The award is aimed at enhancing research and education at all levels. It especially impacts studies correlating molecular structure, orientation and dynamics. Researchers use the MALDI-TOF-MS to study a number of exciting projects. Research will be enabled that is aimed at the development of a comprehensive approach to mass spectrometry imaging that is quantitative and capable of biomolecule identification. MALDI imaging is combined with IR and UV laser ablation sample transfer for liquid chromatography tandem mass spectrometry analysis. In addition, novel approaches to further improve the MALDI technology will be developed. Two new platforms are developed that improve biological imaging. Researchers are investigating the effect of coronal charge patterning on the equilibrium aggregate structure of amphiphilic ionic block copolymers. The impact of structural precision in the properties and functions of discrete linear and branched synthetic polymers are being studied. MALDI is being used to validate recently developed high-resolution and high- speed Raman micro-spectroscopy method to detect lipid modulation and image spatial distribution of lipids in vitro and ex vivo brain tissues. The instrument enables the elucidation of the underlying chemistry of homogeneous lignin depolymerization using a contactless gas phase reactor and upgrade fast pyrolysis of lignin toward formation of biofuel. The instrument is aiding the design, synthesis and study of the photophysical properties and potential applications of new fluorophores that absorb and emit in the visible and near-infrared region of the optical spectrum (400–900 nm). Among the current fluorophores under investigation, boron dipyrromethene (BODIPY) dyes display a rich array of photophysical and optoelectronic properties. Fundamental knowledge will be gained on alkyne metathesis reactions and the creation of design rules toward catalysts with higher activity and broader substrate scope. This includes the design and synthesis of new catalyst and ligand systems and analysis of their reactivities both experimentally and computationally. Additional studies enabled are the developmental competence of in vitro matured oocytes for use in assisted reproductive technologies (ART), the synthesis and application of a specific class of ionic liquid (IL) compounds termed, “group of uniform materials based on organic salts” (GUMBOS), which are used as MALDI matrixes for imaging applications, and uncovering the rules governing changes in electron transfer after encapsulation of redox active molecules in confined spaces. Researchers are studying molecular catalysts containing metal-ligand multiple bonds that are driven by renewable energy for fuel production and industrial applications.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要的研究工具计划，化学研究工具计划和启发竞争性研究（EPSCOR）的既定计划共同资助。来自路易斯安那州立大学的Kermit Murray教授代表该大学4个部门的11位调查人员，正在收购配备有光电码数阵列的矩阵辅助激光解吸 /电离时间质谱仪（MALDI-TOF-MS）。通常，质谱法（MS）是用于识别和表征复杂样品中少量化学物种的关键分析方法之一。 Maldi TOF结合了温和的电离（非常适合产生完整的肽，蛋白质，核酸，碳水化合物，合成聚合物和其他类似尺寸的物种的离子化）和检测模式，该模式在广泛的样品范围内提供了良好的平衡和准确性之间的平衡。该收购能够增强大学和该地区的研究基础设施。该乐器通过与潜水员的学生群体进行动手访问来扩大参与。该仪器还用于外展活动。该奖项旨在增强各级研究和教育。它特别影响了将分子结构，方向和动力学相关的研究。研究人员使用MALDI-TOF-MS研究许多令人兴奋的项目。将启用研究旨在开发质谱成像的综合方法的研究，该方法具有定量性且能够生物分子鉴定的能力。 MALDI成像与IR和紫外线激光消融样品转移结合使用，用于液相色谱串联质谱分析。此外，还将开发出新的改进MALDI技术的新方法。开发了两个新的平台，以改善生物成像。研究人员正在研究冠状电荷模式对两亲离子块共聚物的等效骨料结构的影响。结构精度在离散线性和分支合成聚合物的性质和功能中的影响正在研究。 MALDI被用来验证最近开发的高分辨率和高速拉曼微光谱法，以检测脂质在体外和体内脑组织的脂质调制和图像空间分布。该仪器可以使用非接触式气相反应器阐明均质木质素沉积的基本化学，并升级木质素的快速热解对生物燃料的形成。该仪器正在协助新荧光团的光物理特性以及在光谱的可见和近红外区域（400-900 nm）吸收和发射的新荧光团的设计，合成和研究。在当前正在研究的荧光团中，硼二吡咯甲烯（BODIPY）染料显示出丰富的光学物理和光电特性。基本知识将获得炔烃的回应反应以及对具有较高活性和更广泛底物范围的催化剂的设计规则的创建。这包括新催化剂和配体系统的设计和合成以及对其反应性的分析。 Additional studies enabled are the developmental competence of in vitro matured oocytes for use in assisted reproductive technologies (ART), the synthesis and application of a specific class of ionic liquid (IL) compounds termed, “group of uniform materials based on organic salts” (GUMBOS), which are used as MALDI matrixes for imaging applications, and uncovering the rules governing changes in electron transfer after encapsulation of氧化还原活性分子在狭窄的空间中。研究人员正在研究含有金属配体多种键的分子催化剂，这些键是由可再生能源用于燃料生产和工业应用的驱动的。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛的影响审查标准通过评估来获得的支持。