Infrared Laser Ablation Microsampling for Mass Spectrometry Imaging

用于质谱成像的红外激光烧蚀微量采样

• 批准号: 1709526
• 负责人: Kermit Murray
• 金额: $ 34.84万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1709526&HistoricalAwards=false
• 关键词: Infrared; Laser; Ablation; Microsampling; Mass; Infrared; Laser; Ablation; Microsampling; Mass

This project is funded by the Chemical Measurement and Imaging program of the Chemistry Division. Professors Kermit Murray of Louisiana State University (LSU) and Professor Touradj Solouki of Baylor University are developing techniques for looking at (imaging) biological tissue in order to identify and quantify the biomolecules in the samples. Tissue imaging is often limited to the most abundant compounds and cannot identify important but less abundant molecules that may be key factors for biological function and disease diagnostics. This project uses lasers for direct sampling of tissue as well as precision microdissection for detailed analysis. The ability to localize, identify, and quantify biomolecules in tissue is important to fundamental research and to clinical diagnostics - especially when it is important to monitor the progress of treatment. The outreach program includes a novel, open education initiative creating Web-based resources for education using the visualization tools developed in this research. Educational opportunities are provided to high school, undergraduate, and graduate students. LSU has a strong history of quality mentorship for underrepresented students, especially African Americans. That quality mentorship is continued and advanced in this work. This project is aimed at the development of a comprehensive approach to mass spectrometry imaging that is quantitative and capable of biomolecule identification. Matrix-assisted laser desorption ionization mass spectrometry imaging is combined with infrared laser ablation sample transfer for liquid chromatography tandem mass spectrometry analysis. The objectives are construction of an infrared laser ablation tissue microprobe, combining MALDI imaging with laser ablation sample transfer, and investigation of the fundamental processes of tissue laser ablation for technique optimization. The educational goals support graduate student training at LSU and Baylor and is promoting collaborative interactions between these institutions. This work has societal benefits through the development of a broadly applicable approach to biological imaging at the molecular level with applications. Education and outreach components of this project involve the development of open resources for mass spectrometry education and research. These components include creating collaborative resources for mass spectrometry on Wikipedia and integration of Human Proteome Organization (HUPO) controlled vocabulary with terminology developed by the International Union of Pure and Applied Chemistry.

该项目由化学分部的化学测量和成像程序资助。路易斯安那州立大学（LSU）的Kermit Murray教授和贝勒大学的Touradj Solouki教授正在开发用于研究（成像）生物组织的技术，以识别和量化样品中的生物分子。组织成像通常仅限于最丰富的化合物，无法识别重要但较少的分子，这可能是生物功能和疾病诊断的关键因素。该项目使用激光直接对组织进行采样以及精确的显微解剖来进行详细分析。在组织中定位，识别和量化生物分子的能力对于基础研究和临床诊断很重要，尤其是在监测治疗进展的重要性时。 外展计划包括一项新颖的开放教育计划，使用本研究中开发的可视化工具创建基于网络的教育资源。 向高中，本科生和研究生提供教育机会。 LSU对代表性不足的学生，尤其是非洲裔美国人的质量指导历史有很强的历史。在这项工作中，这种质量指导一直持续并提高。该项目旨在开发一种综合方法来进行质谱成像，该方法具有定量且能够生物分子鉴定的能力。基质辅助激光解吸电离质谱成像与红外激光消融样品转移结合使用，用于液相色谱串联质谱分析。这些目标是构建红外激光消融组织微探针，将MALDI成像与激光消融样品转移相结合，并研究组织激光消融的基本过程以进行技术优化。教育目标支持LSU和Baylor的研究生培训，并正在促进这些机构之间的协作互动。这项工作通过在分子水平上开发了广泛适用的生物成像方法，从而具有社会利益。该项目的教育和外展成分涉及开放质谱教育和研究的开放资源。这些组成部分包括为Wikipedia创建协作资源，以及与国际纯净和应用化学联盟开发的术语的人类蛋白质组组织（HUPO）控制词汇的整合。