Picosecond Infrared Laser Ablation Mass Spectrometry for Rapid Characterization of Biological Tissues

用于快速表征生物组织的皮秒红外激光烧蚀质谱法

• 批准号: RGPIN-2018-04611
• 负责人: ZarrineAfsar, Arash
• 金额: $ 1.75万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=669585
• 关键词: Picosecond; Infrared; Laser; Ablation; Mass

***My overall research program aims to develop ‘needs driven' enabling ‘sensing' technologies to address unmet needs in the biomedical domain. This proposal describes engineering developments of a laser-based sensing molecular analysis platform capable of characterizing organic matter in a few seconds of laser interrogation and analysis time. The platform will include a wide array of applications in the areas of forensic and food science to rapid pathology, the latter being the focus of proposed validation herein as it addresses a clear unmet need. Sensing is achieved with Mass Spectrometry (MS) that can deliver a molecular complexity profile of the sample which, in combination with a library of complexity profiles, can be identified unambiguously and rapidly with MS. ******Ablative laser systems capable of desorptive extraction, to the gas phase, of sample molecular content are currently used in a number of MS based molecular analysis platforms. This proposal explores the utility of a specialized, pulsed mid-infrared (IR) laser system that harbours the most optimal conversion of the impulsively deposited vibrational energy to molecular libration. ******For water-rich samples such as biological tissues, molecular desorption with a Picosecond InfraRed Laser (PIRL) is a highly efficient process due to the strong coupling between libration and vibrational modes of water on this timescale. The bulk of the impulsive energy deposited into the vibrational mode of tissue water molecules is converted into desorption, liberating water and tissue constituent molecules, ejecting them to the gas phase. The process takes place faster than heat transfer from superheated water molecules to the surrounding sample molecules, leaving them intact. ******This proposal describes engineering, development and the integration of a hand held PIRL-MS probe for the specific purpose of rapid molecular fingerprinting of biological tissues and accelerated decision making; a probe that delivers rapidly information regarding tissue type, tissue chemistry, metabolic state, disease state such tumor type, subtype and disease site margins, based on real time analysis of laser liberated tissue chemical content.******Relevant engineering principles will be leveraged to develop an optimized collection and ionization interface, further augmented with statistical analysis methods, and integration with tracking and positioning technologies towards a hand-held molecular profiling tool. Coupling the output of the statistical analysis with 3D positioning systems allows spatially encoded mass spectrometry results (i.e. classifiers) to visualize pathology through an augmented reality display platform. The bundled solution comprising the laser, MS analyzer, molecular fingerprint library, statistical analysis platform and navigation display for spatially encoded MS results will be validated using real world samples.

***我的整体研究计划旨在开发“需求驱动”，使“传感”技术能够满足生物医学领域未满足的需求。该建议描述了基于激光的传感分子分析平台的工程发展，该平台能够在几秒钟内激光询问和分析时间内表征有机物。该平台将包括在法医和食品科学领域中对快速病理学领域的广泛应用，后者是拟议的重点。在此处验证，因为它解决了明确的未满足需求。通过质谱（MS）实现感测，可以提供样品的分子复杂度曲线，并结合使用复杂性曲线的库，可以与MS明确和快速地识别。 *****目前在许多基于MS的分子分析平台中使用了能够解脱提取到气相的烧蚀激光系统。该建议探讨了专门的，脉冲的中红外（IR）激光系统的实用性，该系统具有最佳的脉冲沉积振动能量到分子库的最佳转换。 *****对于诸如生物学时机之类的水样样品，具有皮秒红外激光（PIRL）的分子解吸是一个高效的过程，这是由于库之间的强耦合和在这个时间表上的振动模式。沉积在组织水分子振动模式中的大部分冲动能量被转化为解吸，释放水和组织构造分子，从而将其驱逐到气相。该过程的发生速度比从过热水分子到周围样品分子的热传递快，使它们完好无损。 *****该提案描述了手工持有的PIRL-MS探测器的工程，开发和整合，目的是针对生物时机快速分子指纹和加速决策做出的特定目的；基于激光解放的组织化学含量的实时分析，将探测有关组织类型，组织化学，代谢状态，疾病状态，类型和疾病部位边缘的疾病。****将利用相关的工程原理。 工具。将统计分析的输出与3D定位系统相结合，允许空间编码的质谱结果（即分类器）通过增强现实显示平台可视化病理学。完成激光器，MS分析仪，分子指纹库，统计分析平台和用于空间编码的MS结果的导航显示的解决方案将使用现实世界样本进行验证。