Acquisition of a High-Throughput Proteomics Robotic Workstation for the Cornell University Bioresource Center

为康奈尔大学生物资源中心购置高通量蛋白质组机器人工作站

• 批准号: 0215897
• 负责人: Kelvin Lee
• 金额: $ 24万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0215897&HistoricalAwards=false
• 关键词: Acquisition; Throughput; Proteomics; Robotic; Workstation

A grant has been awarded to Cornell University to acquire a high-throughput robotics platform which will perform two-dimensional protein electrophoresis (2DE) gel spot picking, spot digestion, digest clean-up and spotting to matrix assisted laser-desorption ionization target plates (MALDI) for mass spectrometry (MS). This robotics platform will be housed in and managed by the Cornell University Biotechnology Resource Center (BRC) and it will be accessible by any investigator at Cornell or at any other institution. The Cornell BRC is a shared-use and fee-for-service facility encompassing nine core services and generating $1.5 million in income during FY 2000/2001. The robotic workstation will enhance the capabilities of the core facility by providing a state-of-the-art instrument which will automatically perform many of the laborious steps required in proteomics experiments. Although the definition can be interpreted broadly, 'proteomics', in this case, refers to the ability to measure changes in the protein expression of a given biological system with the goal of relating such changes to various disease states, metabolic states or other features of biological interest. The acquisition of this instrument will have a positive impact on the research programs of many of the investigators at Cornell and at any other institution interested in using this resource.In the field of proteomics, proteins are collected from biological samples of interest with the goal of identifying changes in protein expression that may be interesting. For example, proteins may be collected from various tissue sources with the goal of identifying changes that relate to the presence or absence of a particular disease. After collection, the proteins are often separated into a unique fingerprint of protein expression that serves as a barcode for that particular sample. These fingerprints or barcodes of protein expression can be compared with the goal of identifying changes in this pattern. Once proteins of interest are identified within this barcode, the proteins must be excised from their polymer matrix, digested with chemicals and placed onto metal plates which can be placed inside separate instruments for analysis. These steps of excision, digest and spotting to metal plates is very laborious and the high-throughput proteomics robotic workstation will be able to perform all of these steps automatically.Cornell University is uniquely positioned to benefit from an integrated proteomics robotics platform because of the relatively large number of established laboratories and investigators that are committed to proteomics. This commitment is reflected by the newly established Cornell Proteomics Program which is a faculty-led, University-wide Program. The CPP currently includes eleven core faculty from eight different units at Cornell spanning a number of different Departments, Colleges and Research Facilities. The acquisition of this instrument will have an important impact on several specific federally-funded research projects (NSF, NIH, USDA) which are being run by Proteomics Program faculty. Many of these projects involve graduate student research assistants that are female or from other underrepresented minorities. Further, many of these graduate student research assistants are funded through NSF Graduate Research Fellowships. Further, this instrument may be used in laboratory teaching activities.

已向康奈尔大学（Cornell University）授予了一项赠款，以获取高通量机器人的平台，该平台将执行二维蛋白电泳（2DE）凝胶点拾取，点消化，消化，消化剂清理和斑点以辅助激光吸收电离电离电离目标平板（MALDI）用于质量图（MS）。该机器人技术平台将由康奈尔大学生物技术资源中心（BRC）内置和管理，康奈尔大学或任何其他机构都可以访问它。 Cornell BRC是一家共享用途和收费服务的设施，其中包括九项核心服务，并在2000/2001财年产生了150万美元的收入。机器人工作站将通过提供最先进的仪器来自动执行蛋白质组学实验所需的许多艰辛步骤，从而增强核心设施的功能。尽管可以广泛解释该定义，但在这种情况下，“蛋白质组学”是指测量给定生物系统蛋白质表达的变化的能力，目的是将这种变化与各种疾病状态，代谢状态或生物学兴趣的其他特征联系起来。该仪器的获取将对康奈尔大学的许多研究人员以及对使用该资源感兴趣的任何机构的研究计划产生积极影响。在蛋白质组学领域，蛋白质是从感兴趣的生物样本中收集的，目的是识别蛋白质表达变化的目标，这可能很有趣。例如，可以从各种组织来源收集蛋白质，其目的是确定与特定疾病存在或不存在有关的变化。收集后，蛋白质通常分为蛋白质表达的独特指纹，该指纹是该特定样品的条形码。可以将这些蛋白质表达的指纹或条形码与鉴定该模式变化的目标进行比较。一旦在此条形码中鉴定出感兴趣的蛋白质，就必须从其聚合物基质中切除蛋白质，并用化学物质消化并放置在金属板上，并将其放置在单独的仪器中进行分析。这些切除，摘要和斑点的步骤非常费力，高通量的蛋白质组学机器人工作站将能够自动执行所有这些步骤。康涅尔大学在相对较大的既定实验室和研究人员都可以从蛋白质的综合实验室和研究人员中受益，从而从综合的蛋白质组织机器人平台中受益。新成立的康奈尔蛋白质组学计划反映了这一承诺，该计划是由教师领导的全大学计划。 CPP目前包括来自康奈尔大学八个不同单位的11个核心教师，涵盖了许多不同部门，大学和研究设施。该工具的获取将对由蛋白质组学计划教师运行的几个特定的​​联邦资助研究项目（NSF，NIH，USDA）产生重要影响。这些项目中有许多涉及女性研究助理，或其他代表人数不足的少数族裔。此外，这些研究生研究助理中的许多是通过NSF研究生研究奖学金资助的。此外，该工具可用于实验室教学活动。