Specialized Engineering Research Equipment: An LC - Ion Trap Mass Spectrometer for Protein, Peptide and Small Molecule Characterization

专业工程研究设备：用于蛋白质、肽和小分子表征的 LC - 离子阱质谱仪

• 批准号: 9912435
• 负责人: Kelvin Lee
• 金额: $ 10万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-15 至 2001-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9912435&HistoricalAwards=false
• 关键词: Specialized; Engineering; Research; Equipment; LC

The objective of this request is to acquire a bundled High Performance Liquid Chromatography (HPLC)/LCQ ion trap mass spectrometer for use in several research projects, and for the training of students. The new LQC ion trap mass spectrometers are able to perform amino acid sequencing experiments, DNA sequencing experiments, mapping of peptide phosphorylation sites and oligosaccharide analysis, in addition to more traditional small molecule characterization. The use of this equipment has the following specific aims: (1) to provide core knowledge and training to undergraduate and graduate students on the use of LCQ ion trap mass spectrometry in at least three different bioengineering applications which are detailed in the specific aims 2 to 4; (2) to sequence and characterize gene products for engineering Escherichia coli with enhanced protein secretion via a proteome science strategy and to make this information web-accessible; (3) to better define and understand the relationship between protein glycosylation and environmental conditions in the insect cell baculovirus expression system to achieve complex glycosylation of a model protein; and (4) to characterize polyethylene glycol (PEG)ylated peptides and proteins, and test hypotheses regarding structure and biodistribution of macromolecular drugs.

该请求的目的是获得捆绑的高性能液相色谱（HPLC）/LCQ离子陷阱质谱仪，用于多个研究项目以及对学生的培训。 除传统的小分子表征外，新的LQC离子陷阱质谱仪还能够执行氨基酸测序实验，DNA测序实验，肽磷酸化位点的映射和寡糖分析。 该设备的使用具有以下特定目的：（1）在至少三个不同目标2至4中详细介绍的三种不同的生物工程应用中，向本科和研究生提供核心知识和培训； （2）通过蛋白质组科学策略增强了蛋白质分泌的工程大肠杆菌的测序和表征基因产物，并使此信息可访问； （3）更好地定义和理解昆虫细胞杆状病毒表达系统中蛋白质糖基化与环境条件之间的关系，以实现模型蛋白质的复杂糖基化； （4）表征聚乙二醇（PEG）蛋白质和蛋白质，以及关于大分子药物的结构和生物分布的测试假设。