Ethylene and Pathogen Infection Responses in Tomato and Arabidopsis

番茄和拟南芥的乙烯和病原体感染反应

• 批准号: 0091064
• 负责人: Harry Klee
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2004-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0091064&HistoricalAwards=false
• 关键词: Ethylene; Pathogen; Infection; Responses; Tomato

The interactions of plants and disease-causing pathogens generally fall into two types: compatible and incompatible. Incompatibility leads to resistance while compatibility leads to disease. In the absence of a resistant response, disease symptoms can spread, leading to severe damage or death of the plant. Analysis of mutants unable to synthesize ethylene, salicylic acid (SA) or jasmonic acid (JA), defined a multistage sequence of disease symptom development during a compatible tomato:pathogen interaction. Synthesis of all three phytohormones is essential for complete symptom development. The spread of disease is associated with an ethylene-dependent accumulation of SA. The position of JA within this cascade of events has yet to be determined. We intend to elucidate how these three hormones control symptom development. Experiments will define at a molecular genetic level the roles of ethylene, SA and JA in symptom development in tomato. Through a combination of hormone quantitation and add-backs, the order of action of the three hormones relative to one another will be determined. Microarray analyses will be performed to identify components of the progression from infection to cell death. These experiments will be complemented by work involving Arabidopsis:Xanthomonas interactions. In Arabidopsis, there is differential attenuation of disease symptoms with ethylene insensitive mutants in the various hormone receptors. This result suggests distinct functions for the five ethylene receptors. Molecular and genetic approaches will be used to define the differential responses. These experiments have the potential to identify unique functions for what has heretofore been considered a redundant receptor family.

植物和致病病原体的相互作用通常分为两种类型：兼容和不兼容。不兼容会导致抵抗力，而兼容性导致疾病。在没有抵抗反应的情况下，疾病症状会传播，导致植物的严重损害或死亡。分析无法合成乙烯，水杨酸（SA）或茉莉酸（JA）的突变体定义了兼容番茄症状症状发育的多阶段序列：病原体相互作用。所有三种植物激素的合成对于完全症状的发展至关重要。疾病的传播与乙烯依赖性SA的积累有关。 JA在这一层次的事件中的位置尚未确定。我们打算阐明这三种激素如何控制症状的发展。实验将在分子遗传水平上定义乙烯，SA和JA在番茄症状发育中的作用。通过荷尔蒙定量和添加添加的结合，将确定三种激素相对于彼此的作用顺序。将进行微阵列分析，以识别从感染到细胞死亡的进展的组成部分。这些实验将与涉及拟南芥的工作相辅相成：xanthomonas的相互作用。在拟南芥中，各种激素受体中乙烯不敏感突变体的疾病症状减弱。该结果表明了五个乙烯受体的不同功能。分子和遗传方法将用于定义差异反应。这些实验有可能确定迄今为止被认为是冗余受体家族的独特功能。