Joint NSF/ERA-CAPS: RegulaTomE - Regulating Tomato Quality through Expression

NSF/ERA-CAPS 联合：RegulaTomE - 通过表达调节番茄质量

• 批准号: 1539831
• 负责人: Harry Klee
• 金额: $ 237.42万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1539831&HistoricalAwards=false
• 关键词: Joint; NSF; ERA; CAPS; RegulaTomE

PI: Harry J. Klee (University of Florida-Gainesville) CoPIs: James J. Giovannoni and Zhangjun Fei (USDA-ARS/ Boyce Thompson Institute for Plant Research)ERA-CAPS Collaborators: Cathie Martin (John Innes Centre, Norwich, United Kingdom), Alisdair Fernie (Max Planck Institute for Molecular Plant Physiology, Golm, Germany), Bjoern Usadel (Aachen University, Aachen, Germany), and Dani Zamir (Hebrew University of Jerusalem, Rehovot, Israel) Producers are under pressure to produce more, cheaper food with reduced inputs and impact on the environment. Consumers need and want healthier, better tasting foods than those produced by today's typical production systems focused on quantity rather than quality. This project focuses on tomato, the most economically important fruit/vegetable crop worldwide. The overall goal of this project is to elucidate the genetic mechanisms controlling plant responses to environmental stresses such as drought and to understand how the environment influences the endogenous chemistry of flavor and nutritional qualities in tomato. This research will deliver mechanistic knowledge as well as tangible tools for agronomic and quality improvement of tomato and other crops. Specifically, naturally occurring versions of genes permitting more robust responses to abiotic stress and positively impacting flavor and nutritional quality will be identified and disseminated to public and private breeders so as to inform their selection processes. The effectiveness of these resources and the significance of the knowledge acquired will ensure that the project contributes directly to food security and sustainable fruit cultivation through use of currently untapped genetic information residing in underutilized wild tomato varieties. With regard to outreach and training, the project will provide unique cross-training opportunities for undergraduate and graduate students as well as postdoctoral scientists on both sides of the Atlantic. In addition, the project will provide summer research internships in genomics and bioinformatics for undergraduate students from Fort Valley State, California State University, Fresno and California State University, Sacramento, all minority serving institutions. The twin objectives of this project are to determine the importance of transcriptional regulation of the metabolic pathways defining quality traits in tomato and to identify such transcriptional regulators at the molecular level. The selected quality traits include those determining antioxidant capacity that impacts shelf life and nutritional value as well as those determining fruit flavor. Loci contributing to abiotic stress tolerance will also be identified with the goal of developing more nutritious and sustainable crops. Natural variation available in introgression lines (ILs) resulting from wild species crosses to tomato will be used to assess the importance of transcriptional regulation, identify regulatory genes and assess epigenetic variation. To accomplish these ends, resources that include a genome reference sequence for S. lycopersicoides and metabolite, DNA methylation and transcriptome profiles of IL fruit will be developed and made freely available. Deliverables include regulatory gene identification and new tools for metabolic engineering of fruit quality. This collaborative US-EU project will allow development of tools and resources on a scale unavailable at a national level. All data produced will be freely and continuously shared within the consortium. Specifically, sequence based datasets will be accessible through a consortium database as well as through publicly available data repositories including GenBank, European Bioinformatics Institute (EBI), and the SOL Genomics Network (SGN).

PI：哈里·J·克莱（Harry J. USADEL（亚兴大学，亚尚，德国）和丹尼·扎米尔（以色列耶路撒冷希伯来大学）生产商承受着更多，更便宜的食物，减少了投入和对环境的影响。消费者需要和想要更健康，品尝食品更好，比当今典型的生产系统所生产的食物更加健康，而不是质量。该项目侧重于番茄，这是全球最重要的水果/蔬菜作物。该项目的总体目标是阐明控制植物对环境压力（例如干旱）的反应的遗传机制，并了解环境如何影响番茄风味和营养品质的内源性化学。这项研究将提供机械知识以及切实的工具，以改善番茄和其他农作物的农艺和质量。具体而言，自然发生的基因版本允许对非生物压力的更强大反应，并将积极影响风味和营养质量产生积极影响，并将其传播给公共和私人育种者，以告知他们的选择过程。这些资源的有效性以及所获得的知识的重要性将确保该项目通过使用当前未经利用的野生番茄品种中的目前未开发的遗传信息来直接促进粮食安全和可持续的水果种植。关于宣传和培训，该项目将为本科生和研究生以及大西洋两岸的博士后科学家提供独特的跨培训机会。此外，该项目还将为来自瓦利堡州立大学，加利福尼亚州立大学，弗雷斯诺和加利福尼亚州立大学的萨克拉曼多堡的本科生提供基因组学和生物信息学的夏季研究实习。 该项目的双重目标是确定定义番茄中质量特征的代谢途径的转录调节的重要性，并在分子水平上确定此类转录调节剂。选定的质量特征包括那些确定抗氧化能力的特征，这些抗氧化能力会影响保质期和营养价值以及确定水果风味的抗氧化能力。为非生物胁迫耐受性做出贡献的基因座也将被确定，目的是开发更多营养和可持续的作物。野生物种交叉到番茄产生的渗入线（IL）中可用的自然变异将用于评估转录调控的重要性，识别调节基因并评估表观遗传变异。为了完成这些目的，将开发并免费提供IL水果的DNA甲基化和转录组谱的基因组参考序列，DNA甲基化和转录组谱。可交付成果包括调节基因识别和用于水果质量代谢工程的新工具。这个协作的美国欧盟项目将允许在国家一级无法使用规模上开发工具和资源。所有产生的数据将在财团内自由，连续共享。具体而言，将通过财团数据库以及包括GenBank，欧洲生物信息学研究所（EBI）和SOL基因组学网络（SGN）等公开数据存储库来访问基于序列的数据集。