Functional Genomics of Tomato Fruit Quality: Bridging the Gap between QTLs and Genes

番茄果实品质的功能基因组学：弥合 QTL 和基因之间的差距

• 批准号: 0923312
• 负责人: Harry Klee
• 金额: $ 181.44万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-15 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923312&HistoricalAwards=false
• 关键词: Functional; Genomics; Tomato; Fruit; Quality

PI: Harry J. Klee (University of Florida - Gainsville)CoPIs: Zhangjun Fei and James J. Giovannoni (Boyce Thompson Institute/USDA-ARS) and Denise Tieman (University of Florida - Gainsville)Collaborator: Alisdair Fernie (Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany)Fruits are major components of the human diet, contributing a large portion of vitamins, minerals, antioxidants, and fiber. While flavor and nutrient composition have clear and profound human benefit, they are difficult traits to modify due to their complex metabolic and regulatory pathways. Synthesis of nutritional and flavor compounds is the result of coordinated activity of multiple biochemical pathways, requiring integration of developmental, physiological and environmental cues. Effective manipulation of these traits requires knowledge of the pathways and the regulatory systems that control them. This project exploits the exceptional diversity within the Solanaceae to identify networks regulating synthesis of flavor and nutrition compounds. For many metabolic pathways, all of the genes encoding biosynthetic activities are known. Yet virtually nothing is known about how those genes are regulated to control metabolic output. The focus of this project is on three specific metabolic pathways responsible for synthesis of major flavor and nutrition chemicals: synthesis of folic acid, carotenoids and a family of phenylalanine-derived flavor and fragrance volatiles. In each case multiple, as yet unidentified genetic loci regulating metabolite flux through these pathways have been identified from wild tomato relatives. This project will specifically identify the genes responsible for that regulation and their mechanisms of action. In order to most effectively accomplish these goals, several valuable community resources will be developed. Gene expression profiling of fruit from lines containing defined segments of the wild tomato relative, Solanum pennellii, will be performed, providing quantitative information for gene expression and gene mapping. Also, BAC end sequencing of a S. pennellii library will be performed, facilitating rapid isolation of orthologous sequences by the entire research community. All sequence data will be available through GenBank and all metabolite, gene expression, and SNP data will be accessible through the Tomato Functional Genomics Database (TFGD, www.ted.bti.cornell.edu). All clone and germplasm resources can be ordered online at http://ted.bti.cornell.edu/cgibin/TFGD/order/home.cgi.This research project integrates genomics, metabolomics, informatics, genetics and biochemistry to achieve a deep understanding of how to manipulate important, multigenic quality traits in plants. This systems approach to a problem provides an excellent training environment for undergraduate and graduate students as well as postdoctoral scientists. The project integrates with ongoing highly successful minority training programs at both institutions. The achievable goals of better and healthier foods are highly appealing to students, industry and the public at large.

PI：哈里·J·克莱（Harry J.饮食，贡献大量的维生素，矿物质，抗氧化剂和纤维。尽管风味和营养成分具有明显而深远的人类利益，但由于其复杂的代谢和调节途径，它们很难修改。 营养和风味化合物的合成是多种生化途径协调活性的结果，需要整合发育，生理和环境线索。对这些特征的有效操纵需要了解途径和控制它们的监管系统。该项目利用了甲酸内科中特殊的多样性，以确定调节风味和营养化合物综合的网络。对于许多代谢途径，已知所有编码生物合成活性的基因。然而，实际上，如何调节这些基因来控制代谢输出。该项目的重点是导致主要风味和营养化学物质合成的三种特定代谢途径：叶酸的合成，类胡萝卜素和苯丙氨酸衍生的风味和香气挥发物。在每种情况下，从野生番茄亲戚中鉴定出了未鉴定的遗传基因座通过这些途径来调节代谢物通量的遗传基因座。该项目将专门确定负责该法规的基因及其作用机制。为了最有效地实现这些目标，将开发一些有价值的社区资源。将进行果实的基因表达分析，这些果实将进行包含野生番茄亲戚solanum pennellii的定义片段的线条，提供用于基因表达和基因映射的定量信息。同样，将对彭内氏链球菌库进行BAC结束测序，从而促进整个研究界的直系同源序列的快速分离。 所有序列数据均可通过GenBank和所有代谢物，基因表达和SNP数据可通过番茄功能基因组数据库（TFGD，www.ted.bti.cornell.edu）访问。 所有克隆和种质资源都可以在http://ted.bti.cornell.edu/cgibin/tfgd/ordore/home.cgi.cgi..cgi..this Research项目将基因组学，代谢组学，信息学，信息学，遗传学和生物化学整合到对多种植物的深入了解的多种植物上，将基因组学，信息学和生物化学整合在一起。这种解决问题的系统方法为大学生和研究生以及博士后科学家提供了出色的培训环境。该项目与两个机构的持续成功的少数民族培训计划融为一体。更好，更健康的食物的可实现目标对学生，行业和整个公众都有很高的吸引力。