Acquisition of a Preparative Ultracentrifuge

购买制备型超速离心机

• 批准号: 9808439
• 负责人: Iwao Ojima
• 金额: $ 4.11万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808439&HistoricalAwards=false
• 关键词: Acquisition; Preparative; Ultracentrifuge

This award from the Chemistry Research Instrumentation and Facilities Program (CRIF) will assist the Department of Chemistry of SUNY at Stony Brook in acquiring a preparative ultracentrifuge. The areas of chemistry enhanced by the equipment include: (1) the design and characterization of new magnetic contrast agents for magnetic resonance imaging, (2) the synthesis of coenzyme A and palmitoyl-coenzyme A analogs, (3) the folding of the ribosomal protein L9 and of the E3/E1P domain, (4) the role of protein surface loops and hinges in the reactions catalyzed by triosephosphate isomerase and the interfacial enzyme cholesterol oxidase, (5) the binding site for fertilin-beta in its receptor, (6) the synthesis of polyfunctional organosilanes as inhibitors of aspartic and metalloproteases, (7) the study of the interfacial enzyme phospholipase D and (8) the spectroscopic analysis of the molecular forces leading to catalysis in the reactions catalyzed by enoyl-coenzyme A hydratase and enoyl-acp reductase. The instrument will also be used in two advanced undergraduate laboratories. Ultracentrifugation is a technique used mainly to aid in the isolation and purification of proteins. The capability of chemists to carry out cutting edge research in bioorganic chemistry demands access to state-of-the-art preparative ultracentrifuges.

该奖项由化学研究仪器和设施计划 (CRIF) 授予，将协助纽约州立大学石溪分校化学系购买一台制备型超速离心机。 该设备增强的化学领域包括：(1) 用于磁共振成像的新型磁造影剂的设计和表征，(2) 辅酶 A 和棕榈酰辅酶 A 类似物的合成，(3) 核糖体的折叠蛋白质 L9 和 E3/E1P 结构域，(4) 蛋白质表面环和铰链在磷酸丙糖异构酶和界面酶胆固醇氧化酶催化的反应中的作用， (5) Fertilin-β 在其受体中的结合位点，(6) 作为天冬氨酸和金属蛋白酶抑制剂的多功能有机硅烷的合成，(7) 界面酶磷脂酶 D 的研究以及 (8) 分子的光谱分析导致烯酰辅酶A水合酶和烯酰acp还原酶催化的反应中的催化作用的力。 该仪器还将用于两个高级本科生实验室。 超速离心是一种主要用于帮助分离和纯化蛋白质的技术。 化学家要想在生物有机化学领域开展前沿研究，就需要使用最先进的制备型超速离心机。