Development of a process to produce proteases and lipases by fermentation of compost leachate and agri-food wastes using Bacillus sp. and Rhizobium sp

开发利用芽孢杆菌发酵堆肥渗滤液和农业食品废物来生产蛋白酶和脂肪酶的工艺。

• 批准号: 544999-2019
• 负责人: Hamel, Lucie
• 金额: $ 5.46万
• 依托单位: Collège Shawinigan
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 2
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=721016
• 关键词: Development; process; produce; proteases; lipases

Sani Marc Group, founded in 1969 at Victoriaville, QC, Canada, is a leader in the manufacture and distribution of cleaning and sanitizing products for industry, agri-food, swimming and spa applications. Always concerned about the protection of the environment, Sani Marc arises to meet the needs on the market in cleaning and sanitizing products by optimizing operating conditions and reducing environmental impact.Likewise, the Sani Marc Group is interested in the production of 'green' enzymes obtained by the fermentation of agri-food residues, such as compost leachate and whey. The enzymes of interest are proteases and lipases produced by Bacillus sp and Rhizobium sp. The production of these enzymes using Bacillus is well documented and the development of a bioprocesses using agri-food residues is highly feasible. In the case of using Rhizobium to produce lipases, the project will let to verify if it would be techno-economically feasible.The project will include the optimization of fermentation and enzyme extraction-purification. The formulations of enzymes will be tested in terms of stability and as raw materials for producing cleaning products. The project focuses the zero-waste concept, by exploring the valorization of liquid wastes. The production of green enzymes will consolidate the leading position of Sani Marc Group in North America as an environmentally responsible enterprise. This project will allow the development of new eco-friendly products which could be organic certified.

Sani Marc Group 于 1969 年在加拿大魁北克省维多利亚维尔成立，是工业、农业食品、游泳和水疗应用清洁和消毒产品制造和分销的领导者。 Sani Marc 始终关注环境保护，通过优化操作条件和减少环境影响来满足市场对清洁和消毒产品的需求。同样，Sani Marc 集团对生产“绿色”酶感兴趣。通过农业食品残留物的发酵，例如堆肥渗滤液和乳清。感兴趣的酶是由芽孢杆菌属和根瘤菌属产生的蛋白酶和脂肪酶。使用芽孢杆菌生产这些酶已有充分记录，并且使用农业食品残留物开发生物工艺是高度可行的。在利用根瘤菌生产脂肪酶的情况下，该项目将验证其在技术经济上是否可行。该项目将包括发酵和酶提取纯化的优化。酶制剂将进行稳定性测试以及作为生产清洁产品的原材料的测试。该项目通过探索液体废物的增值来关注零废物概念。绿色酶的生产将巩固Sani Marc集团在北美作为环保企业的领先地位。该项目将允许开发可通过有机认证的新环保产品。