Effects driven assessment and management of complex operating sites

复杂作业场所的效果驱动评估和管理

• 批准号: 505935-2016
• 负责人: Siciliano, Steven
• 金额: $ 9.91万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=696802
• 关键词: Effects; driven; assessment; management; complex

There are many complex operating sites located across Canada. These sites contain a wide variety of chemicals of potential concern, many of which have arisen due to partial breakdown in the soil and water. For most of these chemicals, there are no well-defined safe limits or detailed toxicological information. The purpose of this Collaborative Research and Development proposal is to develop a method to assess the amount, environmental movement, toxicity and risk of complex mixtures of ill-defined pollutants. Working closely with our industrial partners, IPCO, Amec Foster Wheller and the Manitoba Conservation Authority, we will use effects driven assessment to estimate the distribution and risk across a complex operating site. Effects driven assessment uses non-animal based toxicity tests, for example liver cell lines, to estimate how toxic a pollutant mixture is in soil, water and air. Here we propose to extend this effects driven assessment to develop a model of how these complex mixtures of toxicants move through the soil, air and water. Following this, we will then use a variety of cell lines, for example intestinal, liver, lung and kidney cell lines, to identify what components of the mixture are most toxic and how do they cause toxicity. Using a fish animal model, we will then confirm this mode of toxicity using a standard animal design. Finally, we will estimate the risk posed to humans by this complex mixture of toxicants by comparing the response of cells lines to the toxicant mixture to the same cell lines' response to a collection of 30 reference toxicants. These reference toxicants already have animal data associated with them, and thus we will be able to read across from our mixture to these reference toxicants to infer safe exposure levels for humans. At the conclusion of this project, we will have developed a method for Canadian's to assess the risk associated with complex operating sites.

加拿大各地都有许多复杂的运营地点。 这些地点包含各种潜在问题的化学物质，其中许多因土壤和水的破裂而引起了许多化学物质。 对于大多数这些化学物质，没有明确的安全限制或详细的毒理信息。 这项协作研究和发展建议的目的是开发一种评估不确定污染物复杂混合物的数量，环境运动，毒性和风险的方法。 与我们的工业合作伙伴，IPCO，AMEC Foster Wheller和Manitoba保护局紧密合作，我们将使用效果驱动的评估来估算复杂的运营地点的分布和风险。 效应驱动的评估使用非动物基于基于动物的毒性测试（例如肝细胞系）来估计污染物混合物如何在土壤，水和空气中有毒。 在这里，我们建议扩展这种效果驱动的评估，以开发出这些毒物的复杂混合物如何在土壤，空气和水中移动的模型。 之后，我们将使用各种细胞系，例如肠，肝，肺和肾细胞系，以确定混合物的哪些成分最有毒，以及它们如何引起毒性。 然后，使用鱼类动物模型，我们将使用标准动物设计确认这种毒性模式。 最后，我们将通过将细胞线对毒物混合物的反应与相同细胞系的反应与30种参考毒物的收集的相同反应进行比较，从而估计有毒物质的复杂混合物对人类带来的风险。 这些参考有毒物质已经具有与之相关的动物数据，因此我们将能够从混合物到这些参考有毒物质的读物，以推断人类的安全暴露水平。 在该项目结束时，我们将为加拿大人开发一种评估与复杂运营站点相关的风险的方法。