Positronic detection of iron reducing, ammonia oxidizing, microorganisms

铁还原、氨氧化、微生物的正电子检测

• 批准号: 537285-2018
• 负责人: Siciliano, Steven
• 金额: $ 3.28万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=720273
• 关键词: Positronic; detection; iron; reducing; ammonia

Naturally occurring bacteria that live in the soil may be able to solve a pressing problem for prairie agriculture; how to clean up excess fertilizer from spills. These bacteria, called Feammox, were only recently discovered and are very difficult to detect because they live off of iron, carbon dioxide and ammonia. Using newly available isotopes at the Saskatchewan Centre for Cyclotron Science (SCCS), we propose to identify and characterize these Feammox bacteria in prairie soils. To do so, we will develop new methods to estimate nitrogen loss from soil using the positron emitting nitrogen isotope, 13N. Following this, we will use the positron emitting isotope of carbon, 11C, to isolate regions of the soil where Feammox bacteria are active. Identification of the region will extend the capabilities of the specialized PET detector present at the SCCS, the Bio-PET, to be able to detect bacteria present in the soil through the use of advanced image reconstruction technologies. Working closely with Federated Cooperative Limited, we will explore if we can add forms of iron to stimulate ammonia removal from soil. In so doing, we will increase agricultural sustainability by reducing the environmental footprint of increased agricultural productivity.

生活在土壤中的天然细菌或许能够解决草原农业面临的紧迫问题；如何清理溢出的多余肥料。这些被称为 Feammox 的细菌是最近才被发现的，而且非常难以检测，因为它们以铁、二氧化碳和氨为生。我们建议利用萨斯喀彻温省回旋加速器科学中心 (SCCS) 新提供的同位素来识别和表征草原土壤中的这些 Feammox 细菌。为此，我们将开发新方法，使用发射正电子的氮同位素 13N 来估算土壤中的氮损失。接下来，我们将使用碳的正电子发射同位素 11C 来隔离 Feammox 细菌活跃的土壤区域。该区域的识别将扩展 SCCS 中的专用 PET 探测器（Bio-PET）的功能，使其能够通过使用先进的图像重建技术来检测土壤中存在的细菌。我们将与联邦合作有限公司密切合作，探索是否可以添加铁的形式来促进土壤中氨的去除。在此过程中，我们将通过减少农业生产力提高对环境的影响来提高农业的可持续性。