Mapping cell metabolism in tissues: NADPH/NADP+ redox state in the regulation of cell dedifferentiation, proliferation, and survival.

绘制组织中的细胞代谢图：NADPH/NADP 氧化还原状态对细胞去分化、增殖和存活的调节。

• 批准号: RGPIN-2016-06468
• 负责人: Rocheleau, Jonathan
• 金额: $ 2.77万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=628785
• 关键词: Mapping; cell; metabolism; tissues; NADPH

Metabolism critically governs cell differentiation, survival, and proliferation. To fully understand the links between cell metabolism and these varied responses, we need methods that measure metabolic flux with high temporal and spatial resolution. The Rocheleau Lab has a long-standing interest in combining quantitative fluorescence microscopy and microfluidic devices ("islet-on-a-chip") to measure the metabolism of individual beta-cells within living pancreatic islets. Pancreatic islets are a valuable model system of nutrient-stimulated insulin secretion; yet also offer the opportunity to study the plasticity and survival of primary cells within a tissue. Our previous NSERC funding allowed us to develop a spectrally tunable genetically encoded sensor (Apollo-NADP

代谢严格控制细胞分化，生存和增殖。为了充分理解细胞代谢与这些多样反应之间的联系，我们需要测量具有高时间和空间分辨率的代谢通量的方法。 Rocheleau Lab对将定量荧光显微镜和微流体设备（“ iSlet-on-A-A-Chip”）相结合，以测量活胰岛中个体β细胞的代谢。胰岛是一种有价值的胰岛素分泌的宝贵模型系统。但还提供了研究组织中原代细胞的可塑性和存活的机会。我们以前的NSERC资金使我们能够开发出可调的遗传编码传感器（Apollo-NADP