Design and fabrication of a standard to align multicolour TIRF microscopes.

设计和制造校准多色 TIRF 显微镜的标准。

• 批准号: 487070-2015
• 负责人: Rocheleau, Jonathan
• 金额: $ 1.82万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=585921
• 关键词: Design; fabrication; standard; align; multicolour

The proposed project is a collaborative effort between the Rocheleau lab and Spectral Applied Research Inc. ("Spectral"). Spectral is a biotechnology company based in Richmond Hill Ontario that aims to find innovative yet practical solutions to optical and opto-mechanical challenges, thereby producing outstanding value for the scientific, industrial and commercial market place (http://www.spectral.ca/home). They specialize in the research and development of custom optical instrumentation. Spectral requires a simple "in-the-field" method to quantify the performance of Total Internal Refection Fluorescence (TIRF) microscopes. More specifically, they need a standard slide to accurately measure the evanescent field penetration depth in multiple colours. They believe a slide with patterned nanoscale tall channels/features (i.e. channels filled with dye that are shorter than the evanescent field) could act as this standard slide; however, they lack the resources to invest directly in this approach. Dr. Rocheleau has unique expertise in microfluidic device design and quantitative fluorescence microscopy. This combined expertise will allow the Rocheleau lab in collaboration with Spectral staff scientists to fabricate and test the new standard slide. The Rocheleau lab has access to the resources needed to fabricate (Microfluidics Foundry) and test (TIRF microscopes) their designs. Spectral does not have access to these resources and it would be prohibitively expensive to develop them for such a short-term project. This proposal entails nanoimprinting microfluidic channels to pattern channels of varying height and thus provide easy standardization in the field.

拟议的项目是Rocheleau Lab与Spectral Applied Research Inc.（“ Spectral”）之间的合作努力。 Spectral是位于安大略省里士满山的一家生物技术公司，旨在寻找针对光学和光学机械挑战的创新但实用的解决方案，从而为科学，工业和商业市场所产生杰出的价值（http://wwww.spectral.ca/home）。他们专门研究定制光学仪器的研究和开发。光谱需要一种简单的“现场”方法来量化总内部反射荧光（TIRF）显微镜的性能。更具体地说，他们需要标准幻灯片来准确地测量多种颜色的evanscent场渗透深度。他们认为，带有图案化的纳米级高通道/特征（即装满染料的通道比淡淡的田地短）的幻灯片可以充当此标准幻灯片；但是，他们缺乏投资的资源 直接采用这种方法。 Rocheleau博士在微流体设备设计和定量荧光显微镜方面具有独特的专业知识。这种结合的专业知识将使罗切洛实验室与光谱员工科学家合作制造和测试新的标准幻灯片。 Rocheleau Lab可以访问其设计（微流体铸造厂）和测试（TIRF显微镜）所需的资源。光谱无法访问这些资源，为这样一个短期项目开发它们将非常昂贵。该提案需要纳米印刷微印的微流体通道，以使高度变化的模式通道，从而在现场提供易于标准化。