Effect of redox potential on the gene expression of Saccharomyces cerevisiae during very-high-gravity ethanol fermentation

超重力乙醇发酵过程中氧化还原电位对酿酒酵母基因表达的影响

• 批准号: 216816-2013
• 负责人: Lin, YenHan
• 金额: $ 1.6万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=593893
• 关键词: Effect; redox; potential; gene; expression

One of the objectives of the proposal is to study the effect of redox potential on the gene expression of Saccharomyces cerevisiae during very-high-gravity (VHG) ethanol fermentation. To achieve the stated objective, a continuous redox potential-controlled fermentation process will be developed, so that VHG ethanol fermentation can be operated at a specified redox potential level. Once steady state is established, yeast cells are harvested and RNAs are extracted. Microarray technology is then applied to obtain whole gene expression profile under a specified redox potential level. As a result, correlation between gene expression pattern and redox potential is established. The genes modulated by redox potential will be identified and their metabolic functions relating to ethanol production under VHG conditions will be elucidated.

该提案的目标之一是研究氧化还原电位对酿酒酵母在非常高的乙醇发酵过程中酿酒酵母基因表达的影响。为了实现既定的目标，将开发连续的氧化还原电势控制的发酵过程，以便可以在指定的氧化还原电位水平上进行VHG乙醇发酵。一旦建立稳态，就收获酵母细胞并提取RNA。然后，将微阵列技术应用于在指定的氧化还原电位水平下获得整个基因表达谱。结果，建立了基因表达模式与氧化还原电位之间的相关性。将确定由氧化还原电位调节的基因，并将阐明其与乙醇产生有关的代谢功能。