Whole-cell gene and protein expression profilings of saccharomyces cerevisiae grown under different gravity environments

不同重力环境下酿酒酵母的全细胞基因和蛋白质表达谱

• 批准号: 216816-2007
• 负责人: Lin, YenHan
• 金额: $ 1.68万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=477789
• 关键词: Whole; cell; gene; protein; expression

Imbalance of carbon dioxide (CO2) has been considered as the major cause of global warming. Many approaches to reducing CO2 emission have been proposed. Using ethanol to replace a portion of gasoline is one of the promising approaches. With a slight modification of the existing ethanol production process, using a high sugar-content substrate becomes possible. However, feeding with a high sugar-content substrate does not guarantee high ethanol yield, but would lead to yeast cell death. Such an adverse result is attributed to a combined osmotic effect due to high sugar concentration and inhibitory effect due to high ethanol level. The detailed cause of such an adverse outcome is still unclear. The global ethanol production in 2005 was nearly 14 billion liters. A one-percent increase in ethanol productivity would translate to tens of million of dollars in profit. Thus a high ethanol-yield production process is welcome.

二氧化碳（CO2）失衡被认为是全球变暖的主要原因。人们提出了许多减少二氧化碳排放的方法。使用乙醇替代部分汽油是有前途的方法之一。通过对现有乙醇生产工艺进行轻微修改，使用高糖含量底物成为可能。然而，用高糖含量的底物喂养并不能保证高乙醇产量，反而会导致酵母细胞死亡。这种不利结果归因于高糖浓度造成的渗透效应和高乙醇水平造成的抑制效应的综合作用。这种不良结果的详细原因尚不清楚。 2005年全球乙醇产量接近140亿升。乙醇生产率提高百分之一将转化为数千万美元的利润。因此，高乙醇产率的生产工艺是受欢迎的。