Effect of redox potential on the gene expression of Saccharomyces cerevisiae during very-high-gravity ethanol fermentation

超重力乙醇发酵过程中氧化还原电位对酿酒酵母基因表达的影响

• 批准号: 216816-2013
• 负责人: Lin, YenHan
• 金额: $ 1.6万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=523597
• 关键词: Effect; redox; potential; gene; expression

One of the objectives of the proposal is to study the effect of redox potential on the gene expression of Saccharomyces cerevisiae during very-high-gravity (VHG) ethanol fermentation. To achieve the stated objective, a continuous redox potential-controlled fermentation process will be developed, so that VHG ethanol fermentation can be operated at a specified redox potential level. Once steady state is established, yeast cells are harvested and RNAs are extracted. Microarray technology is then applied to obtain whole gene expression profile under a specified redox potential level. As a result, correlation between gene expression pattern and redox potential is established. The genes modulated by redox potential will be identified and their metabolic functions relating to ethanol production under VHG conditions will be elucidated.

该提案的目标之一是研究超重力（VHG）乙醇发酵过程中氧化还原电位对酿酒酵母基因表达的影响。为了实现既定目标，将开发连续氧化还原电位控制的发酵工艺，以便VHG乙醇发酵可以在指定的氧化还原电位水平下进行。一旦建立稳定状态，就收获酵母细胞并提取 RNA。然后应用微阵列技术获得特定氧化还原电位水平下的全基因表达谱。结果，建立了基因表达模式和氧化还原电位之间的相关性。将鉴定受氧化还原电位调节的基因，并阐明它们与 VHG 条件下乙醇生产相关的代谢功能。