Exploring PFpP organometallic nanoparticles for sensing technology

探索用于传感技术的 PFpP 有机金属纳米颗粒

• 批准号: 479146-2015
• 负责人: Wang, Xiaosong
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579962
• 关键词: Exploring; PFpP; organometallic; nanoparticles; sensing

Environmental Bio-Detection Products Inc. (EBPI) in Mississauga is developing sensing techniques for monitoring of a range of environmental contaminants. In order to develop low cost, but highly sensitive and robust sensing devices, techniques to collect analytes effectively and concentrate to sufficient levels to allow detection down to the ug/L range are crucial and remain a significant challenge. Aqueous colloids assembled from metal carbonyl organometallic polymers, PFpP, possess large surface areas per unit mass and offer the promise to serve as ideal materials for sample/analyte collection if their surface can be successfully decorated with analyte binding groups. A second step will require the concentration of these particles containing the bound analytes from a range of complex environmental matricies, e.g. natural waters. This concentration step will be necessary to allow for the detection of the concentrated analytes at environmentally meaningful levels. To address EBPI's specific problems, involving analyte collection and concentration, the PFpP colloids will be decorated with mamaleimide groups. This will in turn allow RNA aptamers to be tethered via thiol-maleimide reactions to the MC colloids. The tethered aptamers are able to complementary bind to specific DNA for target detection. In addition, by taking advantage of the hollow structure of the MC colloids, magnetic nanoparticles will be encapsulated within them. The resultant hybrid particles, after collecting analyses, can be concentrated using magnetic forces allowing for high detection of specific target analytes at environmentally meaningful concentrations (ng to ug/L levels) . Dr. Don Lush with a background in environmental biology, and Dr. Aaron Witham with a background in molecular chemistry, employees of EBPI will participate in directing the research toward rapid initial product commercialization.

位于密西沙加的环境生物检测产品公司 (EBPI) 正在开发用于监测一系列环境污染物的传感技术。为了开发低成本、高灵敏度和鲁棒性的传感设备，有效收集分析物并将其浓缩到足够水平以允许检测低至 ug/L 范围的技术至关重要，并且仍然是一个重大挑战。 由金属羰基有机金属聚合物 PFpP 组装而成的水性胶体，单​​位质量具有较大的表面积，如果其表面可以成功地用分析物结合基团修饰，则有望成为样品/分析物收集的理想材料。第二步需要浓缩这些含有来自一系列复杂环境基质（例如环境基质）的结合分析物的颗粒。天然水域。为了检测对环境有意义的水平的浓缩分析物，该浓缩步骤是必要的。 为了解决 EBPI 的具体问题，包括分析物收集和浓缩，PFpP 胶体将用马来酰亚胺基团进行修饰。这反过来又允许 RNA 适体通过硫醇-马来酰亚胺反应与 MC 胶体结合。系留适体能够与特定 DNA 互补结合以进行目标检测。此外，利用MC胶体的中空结构，磁性纳米粒子将被封装在其中。收集分析后得到的混合颗粒可以利用磁力进行浓缩，从而可以在对环境有意义的浓度（ng 至 ug/L 水平）下对特定目标分析物进行高检测。具有环境生物学背景的 Don Lush 博士和具有分子化学背景的 Aaron Witham 博士，EBPI 的员工将参与指导 研究快速初始产品商业化。