Time-of-flight mass spectrometry of biomolecules using particle and laser induced desorption

使用粒子和激光诱导解吸对生物分子进行飞行时间质谱分析

• 批准号: 38415-2011
• 负责人: Ens, Werner
• 金额: $ 1.6万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=474809
• 关键词: Time; flight; mass; spectrometry; biomolecules

The dramatic developments in gene technology, and the completion of genome sequences for a variety of organisms, including humans, provide unprecedented opportunities for rapid and sensitive determination of the identity and function of proteins. In this field of proteomics, mass spectrometry plays a pivotal role, due to similarly dramatic developments in this field, many of them in physics laboratories. In the late 80s, limited accessible mass range and sensitivity were largely overcome by the invention of two new ionization techniques, matrix-assisted laser desorption/ionization (MALDI), and electrospray ionization (ESI). The 2002 Nobel prize in Chemistry was awarded in part for these developments.

基因技术的戏剧性发展以及包括人类在内的各种生物的基因组序列的完成，为快速和敏感的蛋白质身份和功能提供了前所未有的机会。在这个蛋白质组学领域，质谱法在该领域的类似发展中起着关键作用，其中许多在物理实验室中。在80年代后期，通过两种新的电离技术，基质辅助激光解吸/电离（MALDI）和电喷雾电离（ESI）的发明很大程度上克服了有限的可及质量范围和灵敏度。 2002年诺贝尔化学奖的部分因这些发展而授予。