MRI: Track 1 Acquisition of a Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight Mass Spectrometer for Central Michigan University

MRI：轨道 1 为中密歇根大学采购液相色谱-电喷雾电离-四极杆飞行时间质谱仪

• 批准号: 2320737
• 负责人: Choon Lee
• 金额: $ 46万
• 依托单位: Central Michigan University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320737&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; Liquid; Chromatography

This award is funded by the Major Research Instrumentation Program and the Chemistry Research Instrumentation Program. Professor Choon Young Lee and 7 other faculty (Drs. Benjamin Swarts, Anja Muller, Bradley Fahlman, Anton Jensen, Douglas Swanson, Janice Tomasik and Dale LeCaptain) from the Chemistry and Biochemistry department at Central Michigan University (CMU) are acquiring an Electrospray Ionization (ESI)-Quadrupole Time of Flight (QTOF) Mass Spectrometer (MS) that is equipped with two soft ionization sources (ESI for readily ionizable molecules and atmospheric pressure chemical ionization for not readily ionizable molecules), an HPLC, a Diode Array Detector, and an autosampler. Mass spectrometry (MS) is an essential analytical method used to help identify and characterize unknown chemical species in complex samples through accurate mass measurement. Hence, its availability is vital to chemists who synthesize new molecules. The department’s research strength lies in advanced materials synthesis, such as dendritic molecules, carbohydrates, polymers, and battery materials. Therefore, an MS that can provide a wide range of molecular weight information and that uses soft ionization techniques allowing the intact molecules to be detected without undergoing fragmentation, will facilitate the accurate mass measurements of various large complex molecules made in the department. Acquisition of the state-of-art ESI-QTOF MS will enable researchers in the Department of Chemistry and Biochemistry at CMU to be efficient in their research and train undergraduate/graduate students on a cutting-edge instrument that will help them acquire skills to succeed in post-graduate study or employment. The award of the ESI-QTOF MS is aimed at enhancing research and education at all levels. The ESI-QTOF MS that offers a broad mass detection range, accurate mass, high resolution/sensitivity, and broad experimental capabilities, will facilitate and impact diverse leading-edge research projects at Central Michigan University. It especially impacts areas involving synthesis of new molecules in chemistry, biochemistry and polymer/materials science. The award of an ESI-QTOF instrument will be used in the analyses of (i) novel powerful, biorelevant macromolecular antioxidant dendrons and dendrimers synthesized through chemical reactions; (ii) carbohydrate- and lipid-based chemical probes that are synthesized to study the glycolipid rich mycobacterial cell envelope and understand mycobacterial physiology; (iii) polymers designed for a variety of applications, such as waste-water treatment, skin scaffolds for burn victims, and fuel cell membranes; (iv) new battery materials synthesized for energy storing applications; (v) enzyme-catalyzed reactions between flavins and enes to better understand their mechanisms of action; and (vi) nano-drugs synthesized by conjugating drug molecules to PAMAM dendrimers or encapsulations to overcome the current problems small drug molecules have. The ESI-QTOF MS instrumentation with MS/MS function also will allow the department to perform proteomic and metabolomic studies as well as determine fragmentation/degradation patterns for the large molecules like dendrimers and polymeric materials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要的研究工具计划和化学研究工具计划资助。 Choon Young Lee教授和其他7位教职员工（Benjamin Swarts博士，Anja Muller，Bradley Fahlman，Bradley Fahlman，Anton Jensen，Anton Jensen，Douglas Swanson，Douglas Swanson，Janice Tomasik和Dale Lecaptain和Dale Lecaptain）来自Chemistry and Biocecray Universion（CMU）的化学和生物化学系（CMU） （QTOF）质谱仪（MS）与两个软电离源相等（用于易于电离的分子的ESI和大气压力化学电离，用于不容易离子的分子），一个HPLC，一个二极管阵列检测器和一个自动摄像头。质谱法（MS）是一种基本的分析方法，用于通过准确的质量测量来帮助识别和表征复杂样品中未知化学物种。因此，其可用性对于合成新分子的化学家至关重要。该部门的研究强度在于先进的材料合成，例如树突状分子，碳水合物，聚合物和电池材料。因此，可以提供广泛的分子量信息并使用软电离技术的MS可以在不进行碎片化的情况下检测到完整的分子，这将促进对部门制造的各种大型复合物分子的准确测量。收购最先进的Esi-Qtof MS将使CMU化学和生物化学系的研究人员能够有效地研究他们的研究，并培训本科/研究生的尖端工具，以帮助他们获得成功的技能，以成功地获得研究生研究或就业。 MS的授予旨在增强各个级别的研究和教育。提供广泛的质量检测范围，准确的质量，高分辨率/敏感性和广泛的实验能力的MS，将有助于和影响中央密歇根大学的领先研究项目。它特别影响涉及化学，生物化学和聚合物/材料科学中新分子的合成的领域。 Esi-QTOF仪器的奖励将用于（i）通过化学反应合成的新型强大的生物含量大分子抗氧化剂和树突聚合物的分析； （ii）合成以研究富含糖脂的分枝杆菌细胞包膜并了解分枝杆菌生理学的基于碳水和脂质的化学问题； （iii）专为各种应用而设计的聚合物，例如废水处理，燃烧受害者的皮肤支架以及燃料电池机制； （iv）合成用于储能应用的新电池材料； （v）黄素和ENE之间的酶催化反应，以更好地了解其作用机理； （vi）通过将药物分子与PAMAM树状聚合物共轭或封装来克服当前小药物分子遇到的问题，通过将药物分子结合到PAMAM树状聚合物或封装来合成。具有MS/MS功能的MS仪器的Esi-QTOF仪器还将允许部门进行蛋白质组学和代谢组学研究，并确定大分子（如树突聚合物和聚合物材料）的碎片化/降解模式。该奖项反映了NSF的法定任务，并通过使用基础的Merit和BroadItal和Broadia and Intformia and croctial和广泛的评估来反映出珍贵的支持。