MRI: Track 1 Acquisition of a Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight Mass Spectrometer for Central Michigan University

MRI：轨道 1 为中密歇根大学采购液相色谱-电喷雾电离-四极杆飞行时间质谱仪

• 批准号: 2320737
• 负责人: Choon Lee
• 金额: $ 46万
• 依托单位: Central Michigan University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320737&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; Liquid; Chromatography

This award is funded by the Major Research Instrumentation Program and the Chemistry Research Instrumentation Program. Professor Choon Young Lee and 7 other faculty (Drs. Benjamin Swarts, Anja Muller, Bradley Fahlman, Anton Jensen, Douglas Swanson, Janice Tomasik and Dale LeCaptain) from the Chemistry and Biochemistry department at Central Michigan University (CMU) are acquiring an Electrospray Ionization (ESI)-Quadrupole Time of Flight (QTOF) Mass Spectrometer (MS) that is equipped with two soft ionization sources (ESI for readily ionizable molecules and atmospheric pressure chemical ionization for not readily ionizable molecules), an HPLC, a Diode Array Detector, and an autosampler. Mass spectrometry (MS) is an essential analytical method used to help identify and characterize unknown chemical species in complex samples through accurate mass measurement. Hence, its availability is vital to chemists who synthesize new molecules. The department’s research strength lies in advanced materials synthesis, such as dendritic molecules, carbohydrates, polymers, and battery materials. Therefore, an MS that can provide a wide range of molecular weight information and that uses soft ionization techniques allowing the intact molecules to be detected without undergoing fragmentation, will facilitate the accurate mass measurements of various large complex molecules made in the department. Acquisition of the state-of-art ESI-QTOF MS will enable researchers in the Department of Chemistry and Biochemistry at CMU to be efficient in their research and train undergraduate/graduate students on a cutting-edge instrument that will help them acquire skills to succeed in post-graduate study or employment. The award of the ESI-QTOF MS is aimed at enhancing research and education at all levels. The ESI-QTOF MS that offers a broad mass detection range, accurate mass, high resolution/sensitivity, and broad experimental capabilities, will facilitate and impact diverse leading-edge research projects at Central Michigan University. It especially impacts areas involving synthesis of new molecules in chemistry, biochemistry and polymer/materials science. The award of an ESI-QTOF instrument will be used in the analyses of (i) novel powerful, biorelevant macromolecular antioxidant dendrons and dendrimers synthesized through chemical reactions; (ii) carbohydrate- and lipid-based chemical probes that are synthesized to study the glycolipid rich mycobacterial cell envelope and understand mycobacterial physiology; (iii) polymers designed for a variety of applications, such as waste-water treatment, skin scaffolds for burn victims, and fuel cell membranes; (iv) new battery materials synthesized for energy storing applications; (v) enzyme-catalyzed reactions between flavins and enes to better understand their mechanisms of action; and (vi) nano-drugs synthesized by conjugating drug molecules to PAMAM dendrimers or encapsulations to overcome the current problems small drug molecules have. The ESI-QTOF MS instrumentation with MS/MS function also will allow the department to perform proteomic and metabolomic studies as well as determine fragmentation/degradation patterns for the large molecules like dendrimers and polymeric materials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器项目和化学研究仪器项目的​​ Choon Young Lee 教授和其他 7 名教师（Benjamin Swarts、Anja Muller、Bradley Fahlman、Anton Jensen、Douglas Swanson、Janice Tomasik 和 Dale LeCaptain）资助。中央密歇根大学 (CMU) 化学与生物化学系正在购买电喷雾电离 (ESI)-四极杆飞行时间 (QTOF) 质量光谱仪 (MS) 配备两个软电离源（ESI 用于易电离分子，大气压化学电离用于不易电离分子）、HPLC、二极管阵列检测器和自动进样器 (MS)。用于通过精确的质量测量来帮助识别和表征复杂样品中的未知化学物质的分析方法，因此，其可用性对于合成新分子的化学家至关重要。因此，能够提供广泛分子量信息并使用软电离技术允许检测完整分子而不发生碎片的质谱仪将有助于准确地合成。获得最先进的 ESI-QTOF MS 将使卡耐基梅隆大学化学与生物化学系的研究人员能够高效地进行研究并培训本科生/研究生。 ESI-QTOF MS 旨在帮助他们获得在研究生学习或就业中取得成功的技能，旨在加强各个级别的研究和教育。质量检测范围、精确质量、高分辨率/灵敏度和广泛的实验能力，将促进和影响中央密歇根大学的各种前沿研究项目，特别是涉及化学、生物化学和聚合物/材料科学中新分子合成的领域。 . 授予ESI-QTOF 仪器将用于分析 (i) 通过化学反应合成的新型强大的、生物相关的大分子抗氧化剂树枝状分子和树枝状聚合物；(ii) 合成的碳水化合物和脂质化学探针，用于研究富含糖脂的分枝杆菌细胞包膜。并了解分枝杆菌生理学；(iii) 设计用于各种应用的聚合物，例如废水处理、烧伤患者的皮肤支架和燃料电池膜； (iv) 合成用于能量储存应用的新型电池材料；(v) 黄素和烷烃之间的酶催化反应，以更好地了解其作用机制；(vi) 通过将药物分子与 PAMAM 树枝状聚合物或封装物结合来合成纳米药物，以克服这些问题具有 MS/MS 功能的 ESI-QTOF MS 仪器还将使该部门能够进行蛋白质组学和代谢组学研究以及确定碎片/降解模式。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。