Core B Metabolomics Proteomics

核心 B 代谢组学 蛋白质组学

• 批准号: 9139481
• 负责人: Donald F Becker
• 金额: $ 27.6万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9139481
• 关键词: Address; Alzheimer' s Disease; Applications Grants; Biochemical; Biological; Biology; Cells; Collaborations; Communities; Complex; Coupled; Data Analyses; Development; Diabetes Mellitus; Disease; Ensure; Equipment; Extramural Activities; Faculty; Friedreich Ataxia; Funding; Goals; Heart Diseases; Homeostasis; Hybrids; Investments; Label; Link; Liquid substance; Liver diseases; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Measures; Metabolism; Methods; Modification; Molecular Profiling; Molecular Target; Nuclear Magnetic Resonance; Oxidation-Reduction; Oxidative Stress; Parkinson Disease; Pathogenesis; Phase; Play; Proteins; Proteomics; Publications; Research; Research Personnel; Research Project Grants; Role; Services; Signal Pathway; Students; System; Systems Biology; Techniques; Technology; Tissues; Training; Translations; abstracting; analytical method; arm; base; biological systems; comparative; flexibility; insight; instrument; instrumentation; meetings; member; metabolic profile; metabolome; metabolomics; multiple reaction monitoring; nervous system disorder; novel; oxidative damage; protein expression; protein metabolite; response; signal processing; success; tool

METABOLOMICS AND PROTEOMICS CORE ABSTRACT The Redox Biology Center (RBC) has combined expertise in mass spectrometry (MS) and nuclear magnetic resonance (NMR) to uniquely meet the metabolomic and proteomic needs of RBC members. The central goal of the RBC's Metabolomics and Proteomics Core (Core A) is to provide support for multiple RBC research projects that benefit from the inclusion of MS proteomics/metabolomics and NMR metabolomics. The Core also strives to support research projects involving investigators outside the RBC and conduct research with industrial partners. The Metabolomics and Proteomics Core is having a tremendous impact on publications and extramural grant applications and will continue to play a critical role in the research of RBC investigators in Phase III. In response to the needs of RBC investigators, the RBC proposes to build a research service in the Metabolomics and Proteomics Core that is dedicated to quantitative measurements of key redox markers. The main services provided by the Metabolomics and Proteomics Core in the Phase III funding cycle will be: 1) MS-based target metabolite measurements, protein identification, PTMs, and global proteomic profiling; 2) NMR-based profiling of metabolic flux in cells (bacterial to mammalian); and 3) measurement of cellular redox status and oxidative stress. To continue providing these services and to support the overall aims of the RBC, funds are requested to support the Metabolomics and Proteomics Core in achieving its four specific aims: 1) maintain instrumentation, further develop MS- and NMR-based methods, and provide technical support to aid the RBC and scientific community at large in metabolomics and proteomics research; 2) establish a research service arm of the Core that capitalizes on the state-of-the-art MS equipment and is dedicated to providing robust analytical methods for quantifying oxidative stress markers and redox status; 3) provide preliminary data and analysis to assist in the success of RBC grant proposal submissions, train/educate RBC faculty and students in MS and NMR methods; 4) facilitate collaborations with RBC and outside researchers by expanding support to projects that will benefit by incorporating metabolomic/proteomic approaches. Core success has been bolstered by major investments by UNL, including funds to support the acquisition of a Bruker SolariX 70 Hybrid FTMS System. This instrument is now part of the Core and provides outstanding flexibility in its application and is enabling the Core to expand its research capabilities. The comprehensive operational plan of the Metabolomics and Proteomics Core coupled with UNL's continued support will ensure the long-term sustainability the Core.

代谢组学和蛋白质组学核心摘要 氧化还原生物学中心（RBC）在质谱（MS）和核磁共振（NMR）方面具有综合专业知识，以唯一满足RBC成员的代谢组和蛋白质组学需求。 RBC的代谢组学和蛋白质组学核心（核心A）的核心目标是为多个RBC研究项目提供支持，这些项目受益于MS蛋白质组学/代谢组学和NMR代谢组学。核心还努力支持涉及加拿大皇家银行以外的研究人员并与工业伙伴进行研究的研究项目。代谢组学和蛋白质组学核心对出版物和壁外赠款应用产生巨大影响，并将继续在第三阶段的RBC研究人员的研究中发挥关键作用。为了满足RBC调查人员的需求，RBC建议在代谢组学和蛋白质组学核心中建立研究服务，该研究致力于对关键氧化还原标记的定量测量。 III期资金周期中代谢组学和蛋白质组学核心提供的主要服务将是：1）基于MS的目标代谢物测量，蛋白质鉴定，PTM和全球蛋白质组学分析； 2）基于NMR的细胞中代谢通量的分析（细菌至哺乳动物）； 3）测量细胞氧化还原状态和氧化应激。为了继续提供这些服务并支持RBC的总体目标，要求资金支持代谢组学和蛋白质组学核心，以实现其四个具体目的：1）维护仪器，进一步发展基于MS和NMR的方法，并提供技术支持，以帮助RBC和RBC和RBC和蛋白质组学研究中的科学社区； 2）建立一个核心的研究服务部门，该部门利用最先进的MS设备，并致力于提供可靠的分析方法来量化氧化应激标志物和氧化还原状态； 3）提供初步数据和分析，以帮助RBC赠款提案提交，培训/教育RBC教师以及MS和NMR方法的学生； 4）通过扩大对将通过合并代谢组/蛋白质组学方法来扩大对项目的支持，从而促进与RBC和外部研究人员的合作。核心的成功得到了核心投资的支持，其中包括资金，以支持收购Bruker Solarix 70 Hybrid FTMS系统。该工具现在已成为核心的一部分，并为其应用提供了出色的灵活性，并使核心能够扩展其研究能力。代谢组学和蛋白质组学核心的全面操作计划以及UNL的持续支持将确保长期可持续性成为核心。