The genetic and developmental basis of repeated phenotypic shifts in Penstemon

Penstemon重复表型转变的遗传和发育基础

基本信息

批准号：
9058147
负责人：
Carrie Wessinger
金额：
$ 5.8万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9058147
关键词：
Address Anisotropy Area Basic Science Bees Biological Candidate Disease Gene Cell Count Cell Proliferation Cell Shape Cell Size Cell division Chromosome Mapping Color Complex Computer software Development Developmental Biology Evolution Flowers Future Gene Expression Gene Targeting Genetic Genetic Models Genetic Transformation Genetic Variation Genetic study Genome Genomic DNA Genomic Segment Genomics Genotype Goals Health Histones Human In Situ Hybridization Individual Investigation Kansas Light Light Microscope Maps Methods MicroRNAs Microscopy Morphology Mutation Organ Organism Pathway interactions Pattern Penstemon Phenotype Plants Population Protocols documentation Quantitative Genetics Quantitative Trait Loci Research Research Training Resource Development Resources Role Sequence Analysis Shapes Shotguns Sodium Glutamate Staging Syndrome System Taxon Techniques Testing Time Training Transgenic Organisms Universities Validation Variant base design developmental genetics experience flexibility genetic evolution human disease insight loss of function microscopic imaging next generation sequencing novel professor programs research and development research study tissue culture tool trait

项目摘要

DESCRIPTION (provided by applicant): A major goal of biomedical basic research is to understand the developmental genetic basis of phenotypic variation. Investigations into the genetic basis of repeated phenotypic evolution in emerging model genetic taxa provide an opportunity to investigate whether, and how often, evolution proceeds through similar genetic and developmental changes and whether there are developmental genetic constraints on phenotypic evolution. The proposed research develops a research program designed to investigate the genetic and developmental basis of repeated shifts in floral pollination syndrome, a complex adaptive trait, in the plant genus Penstemon. These initial experiments will provide insights and valuable resources for the development of this research program. In this genus, there have been at least 15 independent evolutionary shifts from the ancestral bee pollination syndrome to a highly stereotypical hummingbird pollination syndrome. The first aim of the proposed research will develop genomic resources and map pollination syndrome divergence for a pair of closely related Penstemon species that differ in their pollination syndrome. Specifically, an F2 mapping population derived from a cross between these two species will be phenotyped for floral traits and genotyped using Multiplexed Shotgun Genotyping, a form of genotyping-by-sequencing. This will enable the construction of a linkage map and identification of quantitative trait loci underlying individual components of pollination syndrome. Genomic DNA from each focal species will be sequenced, assembled, and aligned to the linkage map to yield a set of ordered genome contigs, comprising a valuable resource for future experiments. The second research aim is to characterize the developmental basis of floral morphological divergence between three separate origins of the hummingbird syndrome by comparing cell number, size, and shape in floral organs across developmental stages for each hummingbird syndrome species relative to a related bee syndrome species. Methods for this aim include microscopy and in situ hybridization to assess patterns of histone4 gene expression, which marks active cell division. The final aim is to develop protocols facilitating stable genetic transformation in Penstemon, which will be a valuable tool for the functional verification of candidate genes in future experiments.

描述（由申请人提供）：生物医学基础研究的主要目标是了解表型变异的发育遗传基础。对新兴模型中反复表型进化的遗传基础的研究为研究提供了一个机会，可以通过相似的遗传和发育变化以及是否存在对表型进化的发育遗传约束。拟议的研究开发了一项研究计划，旨在研究植物penstemon植物属的花卉授粉综合征反复转移的遗传和发育基础。这些最初的实验将为该研究计划的制定提供见解和宝贵的资源。在该属中，从祖先蜜蜂授粉综合征到高度定型的蜂鸟授粉综合征至少有15种独立的进化转变。拟议的研究的第一个目的是开发基因组资源和地图授粉综合征的差异，该综合征的差异与其授粉综合征不同。具体而言，将对这两个物种之间的十字架得出的F2映射种群进行表型，并使用多重shot弹枪基因分型（一种基因分型逐个测试形式）进行基因分型。这将使构建链接图并识别授粉综合征个体组成部分的定量性状基因座。将对每个焦点物种的基因组DNA进行测序，组装和对齐与链接图，以产生一组有序的基因组重叠群，其中包括未来实验的宝贵资源。第二个研究的目的是表征蜂鸟综合征的三个独立起源之间的花卉形态差异的发育基础，通过比较相对于相关BEE综合征的每个蜂鸟综合征的细胞数，大小和形状。此目的的方法包括显微镜和原位杂交评估组蛋白4基因表达的模式，该模式标记了活性细胞分裂。最终目的是开发促进Penstemon中稳定遗传转化的方案，这将是在未来实验中对候选基因功能验证的有价值工具。