The genetic and developmental basis of repeated phenotypic shifts in Penstemon

Penstemon重复表型转变的遗传和发育基础

基本信息

批准号：
8845982
负责人：
Carrie Wessinger
金额：
$ 5.42万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8845982
关键词：
Address Anisotropy Area Basic Science Bees Biological Candidate Disease Gene Cell Count Cell Proliferation Cell Shape Cell Size Cell division Chromosome Mapping Color Complex Computer software Development Developmental Biology Evolution Flowers Future Gene Expression Gene Targeting Genetic Genetic Models Genetic Transformation Genetic Variation Genetic study Genome Genomic DNA Genomic Segment Genomics Genotype Goals Health Histones Human Image In Situ Hybridization Individual Investigation Kansas Light Light Microscope Maps Methods MicroRNAs Microscopy Morphology Mutation Organ Organism Pathway interactions Pattern Penstemon Phenotype Plants Population Protocols documentation Quantitative Genetics Quantitative Trait Loci Relative (related person)Research Research Training Resource Development Resources Role Sequence Analysis Shapes Shotguns Sodium Glutamate Staging Syndrome System Taxon Techniques Testing Time Training Transgenic Organisms Universities Validation Variant base design developmental genetics experience flexibility genetic evolution human disease insight loss of function next generation sequencing novel professor programs public health relevance research and development research study tissue culture tool trait

项目摘要

DESCRIPTION (provided by applicant): A major goal of biomedical basic research is to understand the developmental genetic basis of phenotypic variation. Investigations into the genetic basis of repeated phenotypic evolution in emerging model genetic taxa provide an opportunity to investigate whether, and how often, evolution proceeds through similar genetic and developmental changes and whether there are developmental genetic constraints on phenotypic evolution. The proposed research develops a research program designed to investigate the genetic and developmental basis of repeated shifts in floral pollination syndrome, a complex adaptive trait, in the plant genus Penstemon. These initial experiments will provide insights and valuable resources for the development of this research program. In this genus, there have been at least 15 independent evolutionary shifts from the ancestral bee pollination syndrome to a highly stereotypical hummingbird pollination syndrome. The first aim of the proposed research will develop genomic resources and map pollination syndrome divergence for a pair of closely related Penstemon species that differ in their pollination syndrome. Specifically, an F2 mapping population derived from a cross between these two species will be phenotyped for floral traits and genotyped using Multiplexed Shotgun Genotyping, a form of genotyping-by-sequencing. This will enable the construction of a linkage map and identification of quantitative trait loci underlying individual components of pollination syndrome. Genomic DNA from each focal species will be sequenced, assembled, and aligned to the linkage map to yield a set of ordered genome contigs, comprising a valuable resource for future experiments. The second research aim is to characterize the developmental basis of floral morphological divergence between three separate origins of the hummingbird syndrome by comparing cell number, size, and shape in floral organs across developmental stages for each hummingbird syndrome species relative to a related bee syndrome species. Methods for this aim include microscopy and in situ hybridization to assess patterns of histone4 gene expression, which marks active cell division. The final aim is to develop protocols facilitating stable genetic transformation in Penstemon, which will be a valuable tool for the functional verification of candidate genes in future experiments.

描述（由申请人提供）：生物医学基础研究的一个主要目标是了解表型变异的发育遗传基础。对新兴模型遗传分类群中重复表型进化的遗传基础的研究提供了一个机会来研究进化是否以及多久通过类似的遗传和发育变化进行，以及表型进化是否存在发育遗传限制。拟议的研究开发了一个研究计划，旨在调查植物花授粉综合症（一种复杂的适应性特征）重复转变的遗传和发育基础。这些初步实验将为该研究项目的发展提供见解和宝贵的资源。在这个属中，从祖先蜜蜂授粉综合症到高度刻板的蜂鸟授粉综合症，至少有 15 次独立的进化转变。拟议研究的第一个目标是开发基因组资源，并绘制一对密切相关的 Penstemon 物种的授粉综合症差异图谱，这些物种的授粉综合症不同。具体来说，将从这两个物种之间杂交衍生的 F2 作图群体将进行花性状表型分析，并使用多重鸟枪基因分型（一种测序基因分型形式）进行基因分型。这将能够构建连锁图谱并鉴定授粉综合征各个组成部分背后的数量性状基因座。来自每个焦点物种的基因组 DNA 将被测序、组装并与连锁图比对，以产生一组有序的基因组重叠群，为未来的实验提供宝贵的资源。第二个研究目的是通过比较每个蜂鸟综合症物种相对于相关蜜蜂综合症物种在各个发育阶段的花器官的细胞数量、大小和形状，来表征蜂鸟综合症的三个不同起源之间花形态差异的发育基础。实现这一目标的方法包括显微镜和原位杂交，以评估组蛋白 4 基因表达模式，这标志着活跃的细胞分裂。最终目标是开发促进 Penstemon 稳定遗传转化的方案，这将成为未来实验中候选基因功能验证的宝贵工具。