Systems Serology Core

系统血清学核心

基本信息

批准号：
10616544
负责人：
Galit Alter
金额：
$ 29.45万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-12 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10616544
关键词：
Address Adult Affect Antibodies Antibody Response Area Autologous Benchmarking Biological Assay Biophysics Birth Cells Collection Data Development Dimensions Ensure Environment Fingerprint Generations Glycoengineering Goals Human Human Resources Humoral Immunities Immune Immune response Immune system Immunity Immunologics Infant Infant Health Infection Infrastructure Intervention Investigation Knowledge Laboratories Leadership Life Maternal antibody Maternally-Acquired Immunity Monitor Nature Neonatal Newborn Infant Pregnancy Protocols documentation Qualifying Reagent Research Resources Role Running Sampling Serology Standardization System Technical Expertise Technology Therapeutic Training Vaccination Vaccines Work antibody test conditioning high standard improved in utero laboratory experience maternal vaccination neonatal immunity neonate new technology next generation novel programs response success synergism technology platform tool

项目摘要

CORE 1 – SYSTEMS SEROLOGY CORE – ABSTRACT Accumulating data points to dramatic immunologic changes during pregnancy and early life. However, how these immune perturbations alter the immune response to vaccination or to infection have yet to be fully defined. Under Core 1, in depth comprehensive characterization will be performed on the humoral immune response to improve our understanding of the mechanisms by which the immune system changes throughout pregnancy, how these changes affect responses to vaccines and how infants benefit from maternal immunity and in turn respond to vaccines. Specifically, Core 1 will exploit and evolve Systems Serology to broadly and deeply probe the nature of the humoral immune response in a sample sparing manner, via the development of multiplexed-antibody profiling approaches. Additionally Core 1 will develop lower-throughput functional assays to probe the interaction between antibodies and host-innate immune cells, in a fully autologous fashion, to define the overall impact of altered antibody and cellular state in pregnancy and early life. Thus Core 1 will deeply interrogate and define changes in humoral immunity throughout pregnancy, at birth, and throughout early infant life. The Core leader will provide all the expertise, infrastructure, reagents, personnel, and training, to ensure the efficient and successful analysis and deployment of this technology under Projects 1 & 2, as well as support in generating data for Projects 2 & 4. Thus the main role of this Core is to provide the leadership and the technical expertise to ensure that antibody assays are run in a standard fashion, that novel technologies evolve from this Core to support the needs of the overall Consortium, and that the Consortium is kept abreast of the latest developments in assessing antibody responses.

核心 1 – 系统血清学核心 – 摘要越来越多的数据表明，怀孕期间和生命早期发生了巨大的免疫变化，然而，这些变化是如何发生的。免疫扰动会改变对疫苗接种或感染的免疫反应，这一点尚未完全确定。核心1，对体液免疫反应进行深入综合表征，以改善我们对免疫系统在整个怀孕期间发生变化的机制的理解，这些变化是如何发生的变化会影响对疫苗的反应以及婴儿如何从母体免疫力中受益，进而对疫苗作出反应具体来说，核心 1 将利用和发展系统血清学来广泛而深入地探索本质。通过开发多重抗体，以节省样品的方式进行体液免疫反应 Core 1 将另外开发低通量功能分析方法来探测相互作用。以完全自体的方式在抗体和宿主先天免疫细胞之间进行分析，以确定抗体的总体影响因此，核心 1 将深入探讨和定义妊娠期和生命早期的抗体和细胞状态。整个怀孕、出生和婴儿早期体液免疫的变化。将提供所有的专业知识、基础设施、试剂、人员和培训，以确保高效和在项目 1 和 2 下成功分析和部署该技术，以及生成支持项目 2 和 4 的数据。因此，该核心的主要作用是提供领导力和技术专业知识为了确保抗体测定以标准方式运行，新技术从该核心发展到支持整个联盟的需求，并确保联盟及时了解最新进展评估抗体反应。