Omics Core

组学核心

基本信息

批准号：
10608169
负责人：
Xiang Zhang
金额：
$ 29.85万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-10 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10608169
关键词：
Address Applications Grants Attention Award Bioinformatics Biological Biological Assay Centers of Research Excellence Chemicals Chromatography Clinical Research Communities Complex Mixtures Continuing Education Data Data Analyses Data Collection Data Reporting Derivation procedure Development Dimensions Disease Drug Interactions Education Ensure Environment Equipment Fee-for-Service Plans Funding Future Goals Health Sciences Hepatology Human Resources Individual Infrastructure Institution Intervention Studies Leadership Liver Liver Dysfunction Liver diseases Malignant neoplasm of liver Mass Spectrum Analysis Methods Modality Modeling Modification Molecular Evolution Peptides Performance Phase Preparation Privatization Procedures Protein Analysis Protein Fragment Proteins Proteomics Protocols documentation RNA Reagent Research Research Activity Research Personnel Research Support Resources S10 grant Sampling Science Services Standardization Statistical Data Interpretation Techniques Technology Toxicology Training Training and Education Translational Research United States National Institutes of Health Universities career career development clinical infrastructure data sharing design epitranscriptomics experimental study fatty liver disease innovation instrument liver development liver injury meetings member metabolomics method development molecular marker non-alcoholic novel nutrition operation problem drinker programs sample collection small molecule success theories tool toxicant

项目摘要

Abstract: The primary function of OMICS Core (Core B) is to provide members of the University of Louisville Hepatobiology and Toxicology COBRE with the expertise, resources, and facilities needed for metabolomic, proteomic, and bioinformatic analyses of biological samples collected to study liver injury, nutrition, gut:liver interactions, and liver:environment/toxicant/drug interactions. Core B personnel will assist with the design of sample collection protocols, will drive the development and the execution of novel and robust chromatographic methods for sample separation and mass spectrometric methods for the analysis of proteins, peptides, and metabolites with a goal of providing a greater understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction. Core B will ensure uniformity and standardization in sample preparation, method development, instrument performance, data collection, data reporting, and data sharing. Core B endeavors to contribute to the overall success and utility of the COBRE and to enhance the infrastructure and the technical and academic capabilities of the institution as a whole. In addition to its function as a service core, Core B will also promote the education and training of the COBRE members and other biomedical investigators with regards to the state-of-the-art omics technologies; with particular attention to the COBRE junior investigators. Furthermore, Core B will become an independent and self-supporting entity using a set comprehensive approaches that have been already implemented and demonstrated their success in our Phase I COBRE. During the Hepatobiology and Toxicology COBRE Phase II period the goals of Core B will be addressed by the following Specific Aims: 1) To support current COBRE and UofL Health Science Center (HSC) projects. 2) To educate and train mentees and other investigators. 3) To develop new protocols and techniques. 4) To become self-sustaining feature of the COBRE and UofL HSC.

摘要：OMICS Core（Core B）的主要功能是为路易斯维尔大学的成员提供肝生物学和毒理学 COBRE 拥有代谢组学、对收集的生物样本进行蛋白质组学和生物信息分析，以研究肝损伤、营养、肠道：肝脏相互作用，以及肝脏：环境/毒物/药物相互作用。核心B人员将协助设计样品采集协议，将推动新颖且强大的色谱的开发和执行样品分离方法和蛋白质、肽和蛋白质分析的质谱方法代谢物的目标是提供对分子机制进化的更好理解与肝功能障碍相关或参与肝功能障碍。 Core B将确保样品的均匀性和标准化准备、方法开发、仪器性能、数据收集、数据报告和数据共享。 Core B 致力于为 COBRE 的整体成功和实用性做出贡献，并加强基础设施以及整个机构的技术和学术能力。除了其作为服务的功能之外核心，核心B还将促进COBRE成员和其他生物医学的教育和培训最先进组学技术的研究人员；特别关注 COBRE 初级调查人员。此外，Core B 将成为一个独立且自立的实体，使用一套已在我们的阶段实施并证明其成功的综合方法我是科布雷。在肝生物学和毒理学 COBRE 第二阶段期间，核心 B 的目标将由以下具体目标： 1) 支持当前的 COBRE 和 UofL 健康科学中心 (HSC) 项目。 2) 教育和培训受训者和其他研究者。 3）开发新的协议和技术。 4) 成为 COBRE 和 UofL HSC 的自我维持功能。