Omics Core

组学核心

基本信息

批准号：
10625861
负责人：
Xiang Zhang
金额：
$ 38.04万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-15 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10625861
关键词：
Acetylation Affinity Alcoholic Hepatitis Alcoholic Liver Diseases Alcohols Analytical Chemistry Bacteria Bilophila wadsworthia Bioinformatics Biological Biometry Broccoli - dietary Butyrates Cecum Chromatography Complex Mixtures Consumption Data Collection Data Reporting Development Enhancers Ensure Epigenetic Process Ethanol FOXP3 gene Family Feces Functional disorder Ginger Glutathione Goals Heavy Drinking Human Human Resources IL17 gene Immunoglobulin Class Switching Individual Inflammatory Infrastructure Institution Intestines Lactobacillus casei rhamnosus Lipids Liver Liver Dysfunction Mass Spectrum Analysis Measures Mediating Mediator Methods Molecular Evolution Monitor Mus Pathogenicity Pathway interactions Patients Peptides Performance Phosphorylation Plasma Play Post-Translational Protein Processing Preparation Probiotics Production Protein Analysis Protein Fragment Proteins Proteomics Protocols documentation Reaction Research Research Personnel Resolution Resources Role STAT3 gene Sampling Science Stable Isotope Labeling Standardization T-Lymphocyte Techniques Technology Testing Therapeutic Intervention Time Tissue Sample Training and Education Translational Research Tryptophan Universities Variant Volatile Fatty Acids alcohol research data sharing design efficacy evaluation exosome follow-up gut dysbiosis gut microbiota inflammatory milieu instrument interleukin-22 lipid mediator lipidomics liver injury member metabolomics method development microbial multidisciplinary nanoparticle novel novel therapeutic intervention nutrition organ injury pathogenic bacteria prevent protective effect sample collection sex stool sample success teratogenesis transcription factor treatment group

项目摘要

The primary function of OMICS Core is to provide members of the University of Louisville Alcohol Research Center (ULARC) with the expertise, resources, and facilities needed for metabolomic, proteomic, and bioinformatic analyses of biological samples collected to study nutrition, gut flora/intestinal dysfunction and alcohol-induced organ injury, and therapeutic interventions. OMICS Core personnel will assist with the design of sample collection protocols, will drive the development and the execution of novel and robust chromatographic methods for sample separation and mass spectrometric methods for the analysis of proteins, peptides and metabolites with a goal of providing a greater understanding of the evolution of molecular mechanisms that correlate with or participate in liver dysfunction. OMICS Core will ensure uniformity and standardization in sample preparation, method development, instrument performance, data collection, data reporting, and data sharing. OMICS Core endeavors to contribute to the overall success and utility of the ULARC and to enhance the infrastructure and the technical and academic capabilities of the institution as a whole. OMICS Core will also promote the education and training of the ULARC members with regards to the state-of-the-art omics technologies. Furthermore, OMICS Core will become an independent and self- supporting entity using a set comprehensive approaches that have been already been implemented and have demonstrated their success in our current ULARC. The OMICS Core is projected to provide necessary and essential support to all ULARC projects.

OMICS Core 的主要功能是为路易斯维尔大学的成员提供酒精研究中心 (ULARC) 拥有代谢组学、蛋白质组学和代谢组学所需的专业知识、资源和设施对收集的生物样本进行生物信息分析，以研究营养、肠道菌群/肠道功能障碍和酒精引起的器官损伤和治疗干预。 OMICS 核心人员将协助设计样本收集协议，将推动新颖且强大的开发和执行用于样品分离的色谱方法和用于蛋白质分析的质谱方法，肽和代谢物，目的是更好地了解分子的进化与肝功能障碍相关或参与肝功能障碍的机制。 OMICS Core 将确保一致性和样品制备、方法开发、仪器性能、数据收集、数据标准化报告和数据共享。 OMICS Core 致力于为整体成功和实用性做出贡献 ULARC 并加强该机构的基础设施以及技术和学术能力所有的。 OMICS Core 还将促进 ULARC 成员在以下方面的教育和培训：最先进的组学技术。此外，OMICS Core 将成为一个独立、自我的支持实体使用一套已经实施并已实施的综合方法在我们当前的 ULARC 中展示了他们的成功。 OMICS Core 预计将提供必要的和对所有 ULARC 项目的重要支持。