OMICS CORE

组学核心

基本信息

批准号：
8813877
负责人：
Xiang Zhang
金额：
$ 34.63万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-10 至 2021-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8813877
关键词：
Ablation Acetylation Affinity Alcohols Arsenic Binding Bioinformatics Biological Blood Centers of Research Excellence Chemicals Complex Mixtures Curcumin Cysteine DNA Binding Domain Diet Drug Interactions Environment Ethanol Event Feces Gas Chromatography Gel Goals Hepatic Hepatic Stellate Cell High Fat Diet Immunoprecipitation Inductively Coupled Plasma Mass Spectrometry Inflammation Injury Intestines Iodoacetamide Iodoacetic Acid Ions Label Lactobacillus casei rhamnosus Large Intestine Lasers Linoleic Acids Liquid Chromatography Liver Liver diseases Mass Spectrum Analysis Measurement Measures Mediating Metals Methods Methylation Mus Pathology Phenotype Phosphopeptides Phosphorylation Plasma Post-Translational Protein Processing Production Protein Fragment Protein Isoforms Proteins Proteomics Recombinant Proteins Regulation Request for Proposals Research Resolution Role Sampling Science Site Small Intestines Stable Isotope Labeling Steatohepatitis Techniques Technology Time Tissue Sample Tissues Toxicology Toxin Unsaturated Fatty Acids Urine Volatile Fatty Acids Zinc exosome feeding liquid chromatography mass spectrometry liver inflammation liver injury mass spectrometer meetings metabolomics non-alcoholic fatty liver novel nutrition oxidation phosphodiesterase IV protein complex protein metabolite recombinant peptide research study response transcription factor tumor growth two-dimensional zinc-binding protein

项目摘要

High-throughput 'omics analysis platforms are the technologies of choice for analyzing complex mixtures derived from tissues and biological matrices such as liver, feces, intestine, blood and urine. To maximize our capability for deciphering the mechanism and therapy for liver injury, nutrition and gut:liver interactions, and liver:environment/toxin/drug interactions, we propose an OMICS Core in this Hepatobiology and Toxicology COBRE proposal. Our OMICS Core is composed of three major components including Metabolomics Core, Proteomics Core, and Bioinformatics. The overarching goal of the OMICS Core is to support the proposed COBRE research. This goal will be met through application of state of the art separations science and mass spectrometry techniques to analyze research samples containing complex mixtures of metabolites, proteins and protein fragments. This goal is achieved through the following aims: Specific Aim 1: Application of liquid chromatography mass spectrometry (LC-MS), comprehensive two- dimensional gas chromatography time-of-flight mass spectrometry (GCxGC/TOF-MS) and integrated laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) platforms for quantitative analysis of metabolites, metal ions, and metal-protein complex in biological samples. Specific Aim 2: Application of high-resolution multidimensional liquid chromatography mass spectrometry (MDLC-MS) and affinity enrichment methods for targeted protein quantification.

高通量组学分析平台是分析复杂混合物的首选技术来源于组织和生物基质，如肝脏、粪便、肠道、血液和尿液。为了最大化我们的破译肝损伤、营养和肠道：肝脏相互作用的机制和治疗的能力，以及肝脏：环境/毒素/药物相互作用，我们在肝生物学和毒理学中提出了组学核心 COBRE 提案。我们的 OMICS 核心由三个主要部分组成，包括代谢组学核心、蛋白质组学核心和生物信息学。 OMICS 核心的总体目标是支持拟议的 COBRE 研究。这一目标将通过应用最先进的分离科学和质量来实现光谱分析技术用于分析含有代谢物、蛋白质的复杂混合物的研究样品和蛋白质片段。这一目标是通过以下目标实现的：具体目标1：应用液相色谱质谱联用仪（LC-MS），综合两三维气相色谱飞行时间质谱 (GCxGC/TOF-MS) 和集成激光烧蚀电感耦合等离子体质谱 (LA-ICP-MS) 平台，用于定量分析生物样品中的代谢物、金属离子和金属-蛋白质复合物。具体目标2：高分辨率多维液相色谱质谱的应用 (MDLC-MS) 和亲和富集方法用于目标蛋白定量。