Cholinergic modulation of odor responses

气味反应的胆碱能调节

• 批准号: 6407963
• 负责人: CHRISTIANE LINSTER
• 金额: $ 7.85万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6407963
• 关键词: acetylcholine; aldehydes; anticholinergic agent; electrophysiology; electrostimulus; extracellular; fatty acids; frontal lobe /cortex; laboratory rat; neural information processing; neural plasticity; neuroregulation; odors; olfactory lobe; olfactory stimulus; pyramidal cells; sensory discrimination; single cell analysis; stimulus /response

DESCRIPTION (provided by applicant): Understanding the role of neuromodulators for cortical function requires the study of their effects at the level of neural responses, as well as at the level of behavioral responses. Theoretical models of olfactory processing, supported by behavioral lesion experiments, predict that cholinergic modulation in the olfactory system would serve to increase the discrimination between similar olfactory stimuli. In vivo extracellular recording techniques will be employed in this research project to test how the olfactory responses of cells in the olfactory bulb and cortex are modulated by cholinergic inputs. The molecular response spectra of mitral cells in the olfactory bulb and of pyramidal cells in the olfactory cortex are modulated by cholinergic inputs in such a way as to increase the discrimination of similar odorants by these cells. Extracellular recording techniques will be used in the olfactory bulb and cortex of anesthetized rats to analyze the degree of overlap in neural activity patterns in response to series of n-aliphatic aldehydes and n-fatty acids. Electrical stimulation of the horizontal limb of the diagonal band of Broca will be used to activate cholinergic projections to the olfactory bulb and cortex. The comparison of neural response profiles to odorants in the absence and in the presence of the stimulation of cholinergic fibers will serve to determine how changes in cholinergic modulation affect odor responses. The cholinergic nature of the observed effects will be tested by injection of cholinergic antagonists (muscarinic and/or nicotinic). The results from these experiments will help understand the processes underlying impairments in odor processing and learning observed in behavioral studies and will test predictions about the functional role of cholinergic modulation in sensory processing.

描述（由申请人提供）：了解神经调节剂的作用 对于皮质功能，需要研究它们在神经水平上的影响 反应，以及行为反应层面。理论模型 嗅觉处理，在行为损伤实验的支持下，预测 嗅觉系统中的胆碱能调节将有助于增加 区分相似的嗅觉刺激。体内细胞外 本研究项目将采用录音技术来测试 嗅球和皮层细胞的嗅觉反应受以下因素调节 胆碱能输入。二尖瓣细胞的分子响应谱 嗅球和嗅皮层中的锥体细胞的调节 胆碱能输入以增加对类似的区分 这些细胞产生气味。细胞外记录技术将用于 麻醉大鼠嗅球和皮层重叠程度分析 响应一系列正脂肪醛的神经活动模式和 n-脂肪酸。对角线水平肢的电刺激 布罗卡带将用于激活嗅觉的胆碱能投射 灯泡和皮质。神经反应曲线与气味物质的比较 缺乏和存在胆碱能纤维刺激将发挥作用 确定胆碱能调节的变化如何影响气味反应。这 观察到的效应的胆碱能性质将通过注射来测试 胆碱能拮抗剂（毒蕈碱和/或烟碱）。这些结果 实验将有助于了解气味受损的潜在过程 在行为研究中观察到的处理和学习并将测试预测 关于胆碱能调节在感觉处理中的功能作用。