Cholinergic modulation of odor responses

气味反应的胆碱能调节

• 批准号: 6523502
• 负责人: CHRISTIANE LINSTER
• 金额: $ 7.85万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6523502
• 关键词: acetylcholine; aldehydes; anticholinergic agent; electrophysiology; electrostimulus; extracellular; fatty acids; frontal lobe /cortex; laboratory rat; neural information processing; neural plasticity; neuroregulation; odors; olfactory lobe; olfactory stimulus; pyramidal cells; sensory discrimination; single cell analysis; stimulus /response

DESCRIPTION (provided by applicant): Understanding the role of neuromodulators for cortical function requires the study of their effects at the level of neural responses, as well as at the level of behavioral responses. Theoretical models of olfactory processing, supported by behavioral lesion experiments, predict that cholinergic modulation in the olfactory system would serve to increase the discrimination between similar olfactory stimuli. In vivo extracellular recording techniques will be employed in this research project to test how the olfactory responses of cells in the olfactory bulb and cortex are modulated by cholinergic inputs. The molecular response spectra of mitral cells in the olfactory bulb and of pyramidal cells in the olfactory cortex are modulated by cholinergic inputs in such a way as to increase the discrimination of similar odorants by these cells. Extracellular recording techniques will be used in the olfactory bulb and cortex of anesthetized rats to analyze the degree of overlap in neural activity patterns in response to series of n-aliphatic aldehydes and n-fatty acids. Electrical stimulation of the horizontal limb of the diagonal band of Broca will be used to activate cholinergic projections to the olfactory bulb and cortex. The comparison of neural response profiles to odorants in the absence and in the presence of the stimulation of cholinergic fibers will serve to determine how changes in cholinergic modulation affect odor responses. The cholinergic nature of the observed effects will be tested by injection of cholinergic antagonists (muscarinic and/or nicotinic). The results from these experiments will help understand the processes underlying impairments in odor processing and learning observed in behavioral studies and will test predictions about the functional role of cholinergic modulation in sensory processing.

描述（由申请人提供）：了解神经调节剂的作用 对于皮质功能，需要研究其在神经水平上的作用 响应以及行为反应的水平。理论模型 由行为病变实验支持的嗅觉加工预测 嗅觉系统中的胆碱能调节将有助于增加 歧视相似的嗅觉刺激。体内细胞外 该研究项目将采用记录技术来测试 细胞在嗅球和皮质中的嗅觉反应受到调节 胆碱能输入。二尖瓣细胞的分子反应光谱 嗅球的嗅球和嗅皮层中的锥体细胞由 胆碱能输入的方式增加了类似的歧视 这些细胞的气味。细胞外记录技术将在 麻醉大鼠的嗅球和皮层，分析重叠程度 在神经活动模式中，响应一系列的N-醛醛和 n脂肪酸。对角线水平肢的电刺激 Broca乐队将用于激活胆碱能投射到嗅觉 灯泡和皮层。神经反应曲线与气味的比较 不存在并在刺激的情况下胆碱能纤维将服务 确定胆碱能调节的变化如何影响气味反应。这 观察到的效果的胆碱能性质将通过注射测试 胆碱能拮抗剂（毒蕈碱和/或烟碱）。这些结果 实验将有助于了解气味损害的过程 在行为研究中观察到的处理和学习将测试预测 关于胆碱能调节在感觉过程中的功能作用。