Cellular and Molecular Studies of Renal Transport

肾脏运输的细胞和分子研究

• 批准号: 9110961
• 负责人: Michael J. Caplan
• 金额: $ 168.62万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9110961
• 关键词: Address; Animals; Body Fluids; Carrier Proteins; Cells; Collaborations; Collection; Complex; Core Facility; Development; Disease; Electrolytes; Environment; Equipment; Fostering; Goals; Health; Human; Human Resources; Individual; Kidney; Laboratories; Lead; Liquid substance; Mediating; Methods; Molecular; Nature; Pathway interactions; Physiology; Process; Property; Proteins; Recording of previous events; Regulation; Renal function; Renal tubule structure; Research; Research Personnel; Techniques; Training; Transport Process; Urine; Work; innovation; insight; macromolecule; member; microscopic imaging; multidisciplinary; novel; phosphoproteomics; programs; public health relevance; tool

DESCRIPTION (provided by applicant): The composition and volume of the body fluid are determined by the kidney. Thus, the mechanisms through which the kidney senses, responds to and adjusts the composition of the forming urine constitute the principal pathways through which the body controls its internal environment. The central importance of these mechanisms in human health is aptly illustrated by the pathophysiological consequences of their perturbation. Breakdowns in the regulation of renal fluid and electrolyte transport result in a wide variety of serious and common conditions. In order to understand how the composition of the urine, and thus of the body fluid is determined, it is necessary to develop a holistic understanding of all of these processes. It is critical to identify the cellular and molecular mechanisms through which the properties of transport proteins and of the paracellular pathway are determined. In addition, it is necessary to elucidate the cellular machinery that controls these regulatory processes. As has been true for our entire history, the overall goal of this Program Project is to understand the mechanisms underlying renal fluid, electrolyte and macromolecule transport. Towards this end, we use a broad spectrum of techniques to address a continuum of problems ranging from the molecular characterization of individual transport-related proteins to the contribution of these proteins to integrated renal function at the level of single cells, intact tubules, the kidney, andthe whole animal. The specific objectives of these projects together constitute a collaborative and synergistic effort to define the mechanisms that regulate multiple aspects of renal transport. No segment of the renal tubule and none of the transport processes that it mediates work in isolation. By exploring several transport processes simultaneously and through a variety of collaborative approaches, we will be able to generate new insights into these complex inter-relationships. It is only through such a coordinated effort that a systematic understanding of renal function can be generated.

描述（由申请人提供）：体液的组成和体积由肾脏确定。因此，肾脏感官，反应并调整形成尿液的组成的机制构成了人体控制其内部环境的主要途径。这些机制在人类健康中的核心重要性是通过其扰动的病理生理后果来恰当地说明的。调节肾脏流体和电解质转运的分解会导致多种严重和常见的条件。为了了解如何确定尿液的组成以及体液的组成，有必要对所有人建立整体理解 这些过程。确定细胞和分子机制，确定转运蛋白和细胞细胞途径的特性。另外，有必要阐明控制这些调节过程的细胞机制。 正如我们的整个历史所做的那样，该计划项目的总体目标是了解 肾脏流体，电解质和大分子转运的机制。为此，我们使用广泛的技术来解决一系列问题，从单个与传输相关蛋白的分子表征到这些蛋白质对单个细胞，完整小管，肾脏和整个动物的综合肾功能的贡献。这些项目的具体目标共同构成了协作和协同的努力，以定义调节肾脏运输多个方面的机制。没有肾小管的部分，也没有孤立地介导的运输过程。通过同时探索几个运输过程，并通过各种协作方法，我们将能够对这些复杂的相互关系产生新的见解。只有通过这种协调的努力，才能产生对肾功能的系统理解。