Bioreagents & Resources Core

生物试剂

基本信息

批准号：
10713943
负责人：
JOSEPH EDWARD WILLIS
金额：
$ 18.6万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-20 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10713943
关键词：
Address Animals Archives Barrett Esophagus Barrett&apos s carcinogenesis Basic Science Biological Assay Blood Blood specimen Body Fluids Cell Line Clinical Clinical Research Collaborations Collection Color Complex Comprehensive Cancer Center Confidentiality of Patient Information Consultations Custom DNA DNA Library Development Diagnostic Dissection Dysplasia EPHB2 gene Ensure Epithelium Esophageal Adenocarcinoma Esophageal Neoplasms Esophageal Tissue Esophageal injury Esophagectomy Esophagus Faculty Family Family suidae Formalin Freezing Fresh Tissue Funding Genomics Gland Goals High grade dysplasia Histology Human Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization Inherited Institutional Review Boards Investigation Knock-in Knockout Mice Link Medical Records Metaplasia Microdissection Modeling Molecular Morphology Mus Outcome Paraffin Embedding Pathologist Pathology Pathway interactions Patients Play Predisposing Factor Predisposition Preparation Procedures Program Research Project Grants Quality Control RNA Reagent Records Research Personnel Research Project Grants Resource Sharing Resources Role Sampling Schedule Services Signal Transduction Site Specimen Submucosa Susceptibility Gene Technology Test Result Tissue Embedding Tissue Microarray Tissue Procurements Tissue Sample Tissues Translational Research United States National Institutes of Health Work biobank blood fractionation cancer prevention carcinogenesis central database design esophageal carcinogenesis experience follow-up gastrointestinal human tissue injury and repair insight laser capture microdissection meetings mouse model novel novel strategies programs quality assurance repository research study screening single cell sequencing skills success technology/technique tissue archive tissue processing tissue resource transcriptomics wound healing

Address Animals Archives Barrett Esophagus Barrett&apos s carcinogenesis Basic Science Biological Assay Blood Blood specimen Body Fluids Cell Line Clinical Clinical Research Collaborations Collection Color Complex Comprehensive Cancer Center Confidentiality of Patient Information Consultations Custom DNA DNA Library Development Diagnostic Dissection Dysplasia EPHB2 gene Ensure Epithelium Esophageal Adenocarcinoma Esophageal Neoplasms Esophageal Tissue Esophageal injury Esophagectomy Esophagus Faculty Family Family suidae Formalin Freezing Fresh Tissue Funding Genomics Gland Goals High grade dysplasia Histology Human Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization Inherited Institutional Review Boards Investigation Knock-in Knockout Mice Link Medical Records Metaplasia Microdissection Modeling Molecular Morphology Mus Outcome Paraffin Embedding Pathologist Pathology Pathway interactions Patients Play Predisposing Factor Predisposition Preparation Procedures Program Research Project Grants Quality Control RNA Reagent Records Research Personnel Research Project Grants Resource Sharing Resources Role Sampling Schedule Services Signal Transduction Site Specimen Submucosa Susceptibility Gene Technology Test Result Tissue Embedding Tissue Microarray Tissue Procurements Tissue Sample Tissues Translational Research United States National Institutes of Health Work biobank blood fractionation cancer prevention carcinogenesis central database design esophageal carcinogenesis experience follow-up gastrointestinal human tissue injury and repair insight laser capture microdissection meetings mouse model novel novel strategies programs quality assurance repository research study screening single cell sequencing skills success technology/technique tissue archive tissue processing tissue resource transcriptomics wound healing

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项目摘要

PROJECT SUMMARY The Biospecimen and Reagents Core [BRC] provides high quality annotated specimens and expert pathology consultation to enhance the P01 projects. The BR&C CWRU and Duke components have long track records of supporting multi-investigator basic, translational and clinical research relating to multiple aspects of the Barrett's Esophagus [BE] and BE- neoplasia. The BRC is co-lead by two nationally recognized gastrointestinal pathologists with expertise in esophageal pathology who have well established track records in tissue-based translational research. The BRC co-leaders have worked together for many years and have longstanding collaborations with the P01 teams. The BRC components have differencing and complementary resources. Both the CWRU and the Duke components have large bioarchives which have been used to support multiple large scale NIH funded projects for over 20+ years. The BR&C has tissue and blood biospecimens from over 6,200 patients suitable for this P01. The BR&C leverages the Case CCC Shared Resources, the UH Pathology Translational Research Core and the Duke BioRepository & Precision Pathology Center shared resource to maximize efficiencies, utilize their expertise and advanced technologies. For Project 1 the BRC was integral to the discovery of germline abnormalities of VSIG10L as a predisposing factor in some patients with Familial Barrett's Esophagus. The BRC worked with Prj 1 to define the knock in and knock out mouse models which form the basis of this project and identified a multilayer epithelium complex in Prj 1 murine models – recognized as a robust marker of BE changes and linked to BE in humans. For Project 2 the BRC and collaborators developed the novel approach of using pigs as a model for esophageal injury – including the study of esophageal submucosal glands [ESMGs] as a potential site of origin of BE. Inspired by this effort, Prj 2 and the BRC identified and provided sections of ESMGs during screening of multiple esophagectomies and then worked with Prj 2 to immunoprofile the ESMGs. The molecular mechanisms identified formed the basis for the project. For Project 3 the BRC supplied high quality tissue samples and immunohistochemistry expertise in the investigation of EPHB2 signaling in esophageal carcinogenesis used to define potential pathways of development of BE – results of which formed the basis for the project. The BRC, with Core C, will continue to provide support to the P01 investigators and will enhance their projects by supplying quality controlled esophagus tissues, human and animal pathology expertise and facilitate state of the art tissue diagnostics including immunohistochemistry, ISH, tissue microdissection, spatial transcriptomics and single cell sequencing.

项目摘要生物测量和试剂核心[BRC]提供高质量的注释标本和专家病理学咨询以增强P01项目。 BR＆C CWRU和Duke组件具有很长的记录支持与多个方面的多个基础，翻译和临床研究巴雷特的食道和肿瘤。 BRC由两个全国认可的胃肠道共同领导具有良好基于组织的往绩记录的食管病理学专业知识的病理学家翻译研究。 BRC共同领导人已经合作了很多年，并且长期以来长期与P01团队的合作。 BRC组件具有差异化和互补资源。 CWRU和Duke组件都有大型生物构造，用于支持多个大规模NIH资助了20多年的项目。 BR＆C从越过的组织和血液生物测量 6,200例适合此P01的患者。 BR＆C利用CCC共享资源，UH病理转化研究核心和杜克生物座位和精密病理中心共享资源最大化效率，利用其专业知识和高级技术。对于项目1，BRC是发现VSIG10L种系异常不可或缺的一部分因某些家族性巴雷特食管患者的因素。 BRC与PRJ 1合作定义了敲门并淘汰构成该项目基础的小鼠模型并确定了多层上皮复合物在PRJ 1鼠模型中 - 被公认为是变化的强大标记，并与人类有关。对于项目2，BRC和合作者开发了使用猪作为模型的新方法食管损伤 - 包括将食管粘膜粘膜[ESMG]作为潜在原产地的研究是。受这项努力的启发，PRJ 2和BRC在筛选期间确定并提供了ESMG的部分多个食管切除术，然后与PRJ 2合作以免疫ESMG。分子确定的机制构成了项目的基础。对于项目3，BRC提供了高质量的组织样品和免疫组织化学专业知识用来定义的食管癌发生中EPHB2信号的研究用于定义的潜在途径 BA的发展 - 结果构成了项目的基础。 BRC与Core C一起将继续为P01调查人员提供支持，并将增强其项目通过提供质量控制的食道组织，人类和动物病理学专业知识并促进 ART组织诊断包括免疫组织化学，ISH，组织显微减退，空间转录组学和单细胞测序。