Core 1: Biospecimen Core

核心 1：生物样本核心

基本信息

批准号：
10227749
负责人：
JOSEPH EDWARD WILLIS
金额：
$ 23.25万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-14 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10227749
关键词：
African American Archives Barrett Esophagus Biological Assay Biological Markers Blood Blood specimen Center for Translational Science Activities Clinical Clinical Medicine Clinical Trials Collaborations Collection Colon Carcinoma Colonic Neoplasms Comprehensive Cancer Center Confidentiality of Patient Information Consultations Core Facility Custom DNA Data Data Set Development Dissection Ensure Esophageal mucous membrane Esophagus Experimental Designs Experimental Pathology Faculty Formalin Formulation Freezing Fresh Tissue Frozen Sections Generations Genes Glutamine Goals Grant Histology Human Human Resources Immunohistochemistry In Situ Hybridization Individual Information Dissemination Institution Institutional Review Boards Laboratories Link Malignant neoplasm of esophagus Malignant neoplasm of gastrointestinal tract Manuscripts Medical center Metabolism Microdissection Modification Molecular Molecular Analysis Morphology Mus Mutate Neoplasm Metastasis Neoplasms Outcome Oxidoreductase PIK3CA gene Paraffin Paraffin Embedding Participant Pathologic Pathology Patients Pharmacology Pilot Projects Play Procedures Process Prostaglandins Protocols documentation Quality Control RNA Research Research Personnel Research Project Grants Resources Role Sampling Schedule Services Specimen Techniques Technology Test Result Testing Tissue Embedding Tissue Microarray Tissue Procurements Tissue Sample Tissues Translational Research Tumor Suppressor Genes University Hospitals Vimentin Work Writing base biological specimen archives blood fractionation career central database clinical database colon carcinogenesis cost effective design experience follow-up gastrointestinal human tissue insight interest laser capture microdissection meetings member programs prospective research study skills success technology development tissue archive tissue processing translational cancer research translational study

项目摘要

PROJECT SUMMARY/ABSTRACT The Biospecimen Core will continue to provide high quality annotated specimens and expert pathology consultation to enhance the projects of Case GI SPORE investigators. This process will continue the longstanding, successful, collaborations between the members of the Core and the individual project investigators. During the course of these collaborations the Core has developed an extensive biospecimen archive derived from over 13,000 patients. Previously, the Core has supplied high quality annotated specimens leading to the discovery of 15-Prostaglandin Dehydrogenase as an important tumor suppressor gene in colon carcinogenesis – the basis of Project 1; the African American colon cancer 15 gene-panel – the basis of Project 2; methylated vimentin as a biomarker of Barrett's Esophagus – the basis of Project 3; and the discovery of unique glutamine metabolism in PIK3CA mutated colon cancers – the basis of Project 4. The Core also provided significant support to the SPORE's Career Enhancement and Pilot Projects Programs. The Core has an extensive archive of colon neoplasms from over 4,800 patients including 2,292 primary colon cancers of known pathologic stage and databased clinical follow-up as well as a substantial number of cancer metastases. Frozen material from 1053 of these cases is also banked with matched normal controls. The Core also has 350 esophageal cancers and Barrett's esophagus paraffin specimens as well as 134 frozen esophageal specimens. Many of these samples have undergone extensive molecular analysis – enabling sophisticated translational studies. The Core works with the Case Comprehensive Cancer Center to make available to the SPORE a wide variety of techniques and technologies in a cost effective manner. The Core will organize all prospective GI biospecimen procurements and distribution to SPORE investigators as well as provide access to its existing tissue archive resource. The Core will support the projects of this application by identification of tissues of interest to investigators both from the Core archive and from prospective accrual. The Core will provide histopathological quality control for tissue sections, along with project specific morphology case reviews and will oversee and provide longitudinal follow-up of clinical outcomes linked to these tissues. The Core will also provide tissue microarray sections for tissue targets of interest, expertise in immunohistochemistry and in situ hybridization assessment and samples appropriate for specific studies. The primary objective is to provide a resource that contributes significantly to the translational goals and endpoints of the individual projects and to facilitate cooperation between other GI SPOREs.

项目概要/摘要生物样本核心将继续提供高质量的带注释样本和专家病理学旨在加强 Case GI SPORE 调查员项目的磋商将继续进行。核心成员与单个项目之间长期、成功的合作在这些合作过程中，核心开发了广泛的生物样本。此前，Core 已经提供了来自超过 13,000 名患者的高质量注释样本。导致发现 15-前列腺素脱氢酶作为结肠中重要的抑癌基因致癌作用 – 项目 1 的基础；非裔美国人结肠癌 15 基因组 – 项目的基础 2；甲基化波形蛋白作为巴雷特食管的生物标志物——项目 3 的基础和发现； PIK3CA 突变结肠癌中独特的谷氨酰胺代谢——项目 4 的基础。核心还提供了对 SPORE 职业提升和试点项目计划的大力支持。来自 4,800 多名患者的结肠肿瘤的广泛档案，其中包括 2,292 例已知的原发性结肠癌病理分期和数据库临床随访以及大量癌症转移。其中 1053 个病例的材料也与匹配的正常对照一起存储。核心还有 350 个病例。食管癌和巴雷特食管石蜡标本以及 134 份冷冻食管标本。其中许多样品进行了广泛的分子分析——实现复杂的转化 The Core 与 Case 综合癌症中心合作，为 SPORE 提供广泛的研究。核心将以具有成本效益的方式组织所有未来的地理标志。生物样本采购和分发给 SPORE 研究人员，并提供对其现有资源的访问组织档案资源。核心将通过识别感兴趣的组织来支持该应用程序的项目。核心档案和预期应计的研究人员将提供组织病理学信息。组织切片的质量控制，以及项目特定形态学案例审查，并将监督和提供与这些组织相关的临床结果的纵向随访。核心还将提供组织。感兴趣的组织靶标的微阵列切片、免疫组织化学和原位杂交方面的专业知识适合特定研究的评估和样本主要目标是提供资源为各个项目的转化目标和终点做出重大贡献，并促进其他 GI SPORE 之间的合作。