Ambystoma Genetic Stock Center

钝口螈遗传资源中心

• 批准号: 10598309
• 负责人: Stephen Randal Voss
• 金额: $ 11.54万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10598309
• 关键词: Administrative Supplement; Adult; Ambystoma; Ambystoma mexicanum; Award; Biological; Biomedical Research; Breeding; CRISPR/Cas technology; Communities; Cryopreservation; Derivation procedure; Development; Disease; Embryonic Development; Future; Genetic; Goals; Health; Housing; Human; Infrastructure; International; Knock-out; Laboratories; Methods; Modeling; Organ Model; Parents; Population; Protocols documentation; Research; Research Personnel; Resources; System; Transgenic Organisms; Translating; equipment acquisition; genome editing; mutant; organ regeneration; sperm cryopreservation; tissue repair; tool

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC houses approximately 880 adults in aquatic recirculating systems and static bowls to sustain a captive, wildtype breeding population and a small number of mutant and transgenic lines. Several dozen axolotl transgenic and knock-out axolotl lines have been created by the axolotl community in the past couple of years and more lines are anticipated in the future. The AGSC does not have enough tanks or infrastructure to house all adult breeders or newly created lines as living stocks. An effort is underway to develop a sperm cryopreservation protocol so that axolotl lines can be managed as both living stocks and cryopreserved stocks. Until the sperm cryopreservation protocol is developed and shown to reproducibly allow for the re-derivation of axolotl lines from frozen sperm, there is critical need to increase the number of tanks in the AGSC. This Administrative Supplement will be used to purchase two recirculating systems that will add 240 tanks to the AGSC. The addition of these new tanks to the AGSC will provide infrastructure that is critically needed to sustain lines for the axolotl community.

项目概要 Ambystoma 遗传库存中心 (AGSC) 是一个 P40 研究资源，负责分发 实验室蝾螈（Ambystoma mexicanum）向全国的研究人员和教育工作者提供 国际上。 AGSC 在水生再循环系统中容纳了大约 880 名成年人 和静态碗来维持圈养的野生型繁殖种群和少量 突变体和转基因系。数十个蝾螈转基因和敲除蝾螈品系已 在过去的几年里，蝾螈社区创建了更多的产品线 预计在未来。 AGSC 没有足够的储罐或基础设施来容纳所有 成年饲养者或新创建的品系作为活畜。正在努力培育精子 冷冻保存协议，以便蝾螈系可以作为活体种群和 冷冻保存的库存。直到精子冷冻保存方案被开发出来并被证明 可重复地允许从冷冻精子中重新衍生蝾螈系，迫切需要 增加 AGSC 中的坦克数量。本行政补充文件将用于 购买两个再循环系统，将为 AGSC 添加 240 个储罐。这些的添加 AGSC 的新坦克将为维持线路提供急需的基础设施 蝾螈社区。