Ambystoma Genetic Stock Center

钝口螈遗传资源中心

• 批准号: 10457068
• 负责人: Stephen Randal Voss
• 金额: $ 11.8万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10457068
• 关键词: Adolescent; Adult; Ambystoma; Ambystoma mexicanum; Animals; Area; Arts; Awareness; Biological; Biomedical Research; Body part; Brain; Breeding; Businesses; Cellular biology; Collaborations; Collection; Communities; Computers; Cryopreservation; Custom; Development; Disease; Embryo; Embryonic Development; Ensure; Environment; Evolution; Funding; Future; Genes; Genetic; Genome; Genomics; Health; Heart; Hour; Human; IACUC; Informatics; Infrastructure; Institution; International; Jaw; Kentucky; Knock-out; Life; Limb structure; Louisiana; Medicine; Methods; Modeling; Natural regeneration; Neurobiology; Newsletter; Online Systems; Organ Model; Physiology; Play; Population; Practice Management; Procedures; Protocols documentation; Reproducibility; Research; Research Personnel; Research Support; Resources; Retina; Role; Salamander; Science; Shipping; Site; Spinal Cord; System; Tail; Technology; Time; TimeLine; Tissues; Transgenic Organisms; Translating; United States National Institutes of Health; Universities; Vertebrates; Work; animal resource; college; cost; experience; genetic resource; knowledge base; learning strategy; meetings; operation; organ regeneration; preservation; single-cell RNA sequencing; sperm cell; sperm cryopreservation; tissue repair; user-friendly; web site; welfare

OVERALL COMPONENT - PROJECT SUMMARY This application seeks support to continue operations of the Ambystoma Genetic Stock Center (AGSC) at the University of Kentucky (UK). The AGSC is the only federally funded resource center that distributes Mexican axolotls (Ambystoma mexicanum), a salamander that provides living materials in support of biomedical research nationally and internationally. Most notably, axolotls are unique among vertebrates in being able to regenerate numerous tissues and body parts. These include the spinal cord, limbs, jaw, retina, brain, heart, and tail, all of which can be regenerated at any point during embryonic, larval, and adult life. Also, axolotls are used by researchers that work in additional areas, including neurobiology, ecotoxicology, development, physiology, cell biology, genetics, genomics, and evolution. This is an exciting time for the axolotl as it’s incredibly large genome (32 Gb) was recently sequenced and assembled. New and assessable resources are allowing cutting edge technologies like gene-editing and single cell RNA sequencing to be performed for the first time, approaches that are rapidly accelerating discoveries using axolotl models. In just the past 4 years, NIH support for axolotl research has tripled and there has been a significant increase in transgenic and knock- out lines in the community. PI’s in the salamander community recently came together to discuss future opportunities and challenges, and the enabling role the AGSC will play in moving community efforts forward. During the previous funding period, the AGSC moved internally from the College of Arts and Sciences to the College of Medicine (COM) at UK. This move has strengthened the AGSC in several significant ways that clearly demonstrate long term commitment by the host institution for this irreplaceable resource. Specifically, funding was made available to replace re-circulating systems and create a more user friendly website to facilitate axolotl stock purchasing and more efficiently disseminate information that is useful for working with axolotls. Additionally, COM-UK has extensive experience working with university core research centers and thus provides a more stable administrative environment to support AGSC business activities and to better ensure regulatory compliance and animal health and welfare. Over the next 5 years, the AGSC will build upon current momentum by maintaining and generating high quality, axolotl stocks that are needed by a growing number of NIH-funded investigators. Husbandry and management practices will be modified to increase availability of post-embryonic stocks that are seeing increasing demand by researchers. Also, transgenic and knock-out stocks will be prioritized for import into the AGSC and cryopreservation methods will be developed to preserve and more efficiently manage stocks. The AGSC will continue to serve as an informatics hub where investigators obtain information about the collection, technical procedures, potential collaborators, and research findings. Overall, the project will ensure distribution and long-term sustainability of axolotl resources to NIH-funded investigators. !

总体组成部分 - 项目摘要 本申请寻求支持 Ambystoma 遗传库存中心 (AGSC) 继续运营 肯塔基大学（英国）是唯一一个由联邦资助的墨西哥资源中心。 蝾螈（Ambystoma mexicanum），一种为生物医学提供生命材料的蝾螈 最值得注意的是，蝾螈在脊椎动物中是独一无二的。 再生许多组织和身体部位，包括脊髓、四肢、下巴、视网膜、大脑、心脏、 和尾巴，所有这些都可以在胚胎、幼虫和成虫生命的任何阶段再生。 供其他领域的研究人员使用，包括神经生物学、生态毒理学、发展、 对于蝾螈来说，这是一个激动人心的时刻，包括生理学、细胞生物学、遗传学、基因组学和进化论。 最近对令人难以置信的大基因组（32 Gb）进行了测序和组装，新的和可评估的资源。 允许执行基因编辑和单细胞 RNA 测序等尖端技术 首次使用蝾螈模型快速加速发现的方法，在过去的四年里， 美国国立卫生研究院 (NIH) 对蝾螈研究的支持增加了两倍，转基因和基因敲除研究也显着增加。 社区中的 PI 最近聚集在一起讨论未来。 机遇和挑战，以及 AGSC 在推动社区努力向前发展方面将发挥的促进作用。 在上一个资助期间，AGSC 从文理学院内部转移到了 英国医学院 (COM) 此举在几个重要方面加强了 AGSC： 明确表明主办机构对这一不可替代资源的长期承诺， 提供资金来更换再循环系统并创建一个更加用户友好的网站 促进蝾螈库存购买并更有效地传播对合作有用的信息 此外，COM-UK 拥有与大学核心研究中心合作的丰富经验。 从而提供更稳定的行政环境来支持 AGSC 的业务活动并更好地开展业务 AGSC 将在未来 5 年内继续致力于确保监管合规性以及动物健康和福利。 通过维持和产生不断增长的动物所需的高质量蝾螈种群来维持当前的势头 NIH 资助的研究人员数量将得到修改，以增加饲养和管理实践。 研究人员对胚胎后种群的需求也不断增加。 淘汰库存将优先进口到 AGSC，并将开发低温保存方法 AGSC 将继续作为信息中心，保护和更有效地管理库存。 调查人员获取有关收集、技术程序、潜在合作者的信息，以及 总体而言，该项目将确保蝾螈资源的分配和长期可持续性。 NIH 资助的研究人员。 ！