Administrative Supplement: Ambystoma Genetic Stock Center

行政补充：Ambystoma 遗传库存中心

基本信息

批准号：
10806471
负责人：
Stephen Randal Voss
金额：
$ 11.77万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-01 至 2024-02-29
项目状态：
已结题

项目摘要

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC houses approximately 880 adults and several thousand larvae/juveniles in aquatic recirculating systems and static bowls to sustain a captive, wildtype breeding population and a growing number of mutant and transgenic lines. To ensure the health of axolotls within the AGSC and their reproducibility in biological experiments it is necessary to follow rigorous husbandry guidelines. The most critical variable in axolotl husbandry is water quality. Water quality must be extremely high to ensure normal axolotl development, growth and reproductive capacity, and to eliminate disease risks associated with pathogens. The AGSC maintains high water quality by ensuring a constant and readably available water supply and rigorous sanitary procedures to ensure the cleanliness of bowls and tanks. Tank and bowl sanitation is a labor-intensive process that is primarily done by hand, and to a lesser extent using an outdated, commercial restaurant dishwasher that is rapidly becoming non-functional. This Administrative Supplement will be used to purchase two Calypso - Aquatic Cabinet Washer systems that are designed to efficiently and effectively clean biological agents and associated biofilms from aquatic organism tanks and bowls. The addition of these new washer systems will provide infrastructure that is critically needed to maintain high water quality and sanitary procedures, increase efficiency of axolotl husbandry, and generate healthy and reproducible axolotl lines for biomedical researchers.

项目概要 Ambystoma 遗传库存中心 (AGSC) 是一个 P40 研究资源，负责分发实验室蝾螈（Ambystoma mexicanum）向全国的研究人员和教育工作者提供国际上。 AGSC 可容纳大约 880 名成年人和数千名水生再循环系统和静态碗中的幼虫/幼虫，以维持圈养的野生型育种群体和越来越多的突变体和转基因品系。为了保证身体健康 AGSC 内蝾螈的数量及其在生物实验中的再现性，有必要遵循严格的饲养准则。蝾螈饲养中最关键的变量是水质量。水质必须极高才能保证蝾螈的正常发育、生长和生殖能力，并消除与病原体相关的疾病风险。 AGSC 通过确保持续且易于使用的供水来保持高水质严格的卫生程序，确保碗和水箱的清洁。水箱和碗环境卫生是一个劳动密集型过程，主要由手工完成，其次是手工完成使用过时的商用餐厅洗碗机，该洗碗机很快就无法使用。本行政补充文件将用于购买两台 Calypso - 水生柜式洗衣机旨在高效且有效地清洁生物制剂和相关产品的系统来自水生生物池和碗的生物膜。添加这些新的清洗系统将提供维持高水质和卫生所急需的基础设施程序，提高蝾螈饲养效率，并产生健康且可重复的生物医学研究人员的蝾螈系。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Systemic cell cycle activation is induced following complex tissue injury in axolotl.

蝾螈复杂的组织损伤后会诱导系统细胞周期激活。

DOI：
发表时间：
2018-01-15
期刊：
影响因子：
2.7
作者：
Johnson, Kimberly;Bateman, Joel;DiTommaso, Tia;Wong, Alan Y;Whited, Jessica L
通讯作者：
Whited, Jessica L

3D visualization of macromolecule synthesis.

大分子合成的 3D 可视化。

DOI：
发表时间：
2020
期刊：
影响因子：
7.7
作者：
Duerr, Timothy J;Comellas, Ester;Jeon, Eun Kyung;Farkas, Johanna E;Joetzjer, Marylou;Garnier, Julien;Shefelbine, Sandra J;Monaghan, James R
通讯作者：
Monaghan, James R

Genetic basis for an evolutionary shift from ancestral preaxial to postaxial limb polarity in non-urodele vertebrates.

非有尾脊椎动物从祖先轴前肢极性到轴后肢极性进化转变的遗传基础。

DOI：
发表时间：
2021-11-22
期刊：
影响因子：
0
作者：
Trofka, Anna;Huang, Bau;Zhu, Jianjian;Heinz, William F;Magidson, Valentin;Shibata, Yuki;Shi, Yun;Tarchini, Basile;Stadler, H Scott;Kabangu, Mirindi;Al Haj Baddar, Nour W;Voss, S Randal;Mackem, Susan
通讯作者：
Mackem, Susan

Axolotl Metabolism: Measuring Metabolic Rate.

蝾螈代谢：测量代谢率。