Taste responses in defined cell types in gustatory cortex

味觉皮层中特定细胞类型的味觉反应

• 批准号: 9055411
• 负责人: John D Boughter
• 金额: $ 22.8万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2017-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9055411
• 关键词: Address; Amygdaloid structure; Anorexia; Area; Behavioral; Biological Assay; Brain region; Calcium; Calcium-Binding Proteins; Cells; Code; Complex; Coronary Arteriosclerosis; Data; Data Set; Diabetes Mellitus; Discrimination; Dorsal; Electrophysiology (science); Evaluation; Goals; Health; Human; Hypertension; Image; Individual; Injection of therapeutic agent; Interneurons; Label; Lateral; Learning; Mammals; Measures; Modeling; Mus; Neurons; Obesity; Oral cavity; Pattern; Play; Population; Positioning Attribute; Publishing; Pyramidal Cells; Rattus; Reporter; Reporting; Research; Rodent; Role; Sodium Chloride; Specificity; Stimulus; Surface; System; Taste Perception; Techniques; Testing; Thalamic structure; Tracer; Transgenic Mice; Variant; Viral; Virus; awake; calcium indicator; calretinin; cell type; extracellular; hedonic; hippocampal pyramidal neuron; in vivo; learned behavior; public health relevance; receptive field; research study; response; sensory system; taste stimuli; taste system; two-photon

 DESCRIPTION (provided by applicant): Ingestive decisions play a key role in a number of human conditions including obesity, diabetes, anorexia, hypertension, and coronary artery disease. One of the most important factors regulating these decisions is the sense of taste. The gustatory cortex in mammals has been shown to be involved in taste learning and behavior, and electrophysiological studies have shown that taste-activated neurons in this area tend to be broadly tuned (with regard to taste quality), multi-modal, and modulated by behavioral state. However, it is not clear how taste quality itself is encoded in the GC, and whether stimulus tuning in individual neurons varies as a function of cell type or functional connection. Two-photon (2P) imaging is a technique that allows for the simultaneous recording of taste-evoked responses in a large numbers of neurons in vivo. However, only a couple of 2P studies in the taste system have been published to date. We will combine 2P imaging with specific identification of cell types, accomplished by either Cre-dependent expression or retrograde labeling. Collectively, these experiments will test the hypothesis that taste sensitivities and tuning profile vary in GC neurons as a function of cortical depth, cell type (pyramidal cell vs. interneuron) or projection pattern. Aim 1 will investigate compare taste responses in Thy1-expressing pyramidal cells and Calretinin-expressing interneurons to the greater population of all labeled cells in GC. Aim 2 will investigate taste responses in GC neurons retrogradely labeled from either gustatory thalamus or basolateral amygdala.

 描述（由适用提供）：摄取决策在许多人类状况中起着关键作用，包括肥胖，糖尿病，厌食症，高血压和冠状动脉疾病。控制这些决定的最重要因素之一是品味感。哺乳动物中的味性皮质已被证明与味觉学习和行为有关，并且电生理研究表明，该地区的味觉激活神经元倾向于广泛调整（关于味道质量），多模式，并受到行为状态的调节。但是，尚不清楚GC中的味道质量本身是如何编码的，并且单个神经元中的刺激调节是否随细胞类型的函数或功能连接而变化。两光子（2p）成像是一种允许在体内大量神经元中同时记录味觉诱发反应的技术。但是，迄今为止，味觉系统中只有几个2P研究。我们将通过CRE依赖性表达或逆行标记来结合2P成像与细胞类型的特定识别。总的来说，这些实验将检验以下假设，即在GC神经元中，随着皮质深度，细胞类型（金字塔细胞与中间神经元）或投影模式的函数而在GC神经元中有所不同。 AIM 1将研究比较表达THY1的锥体细胞和表达钙网蛋白的中间神经元与GC中所有标记细胞中较大人群的味觉反应。 AIM 2将研究从味觉丘脑或底层杏仁核逆行标记的GC神经元中的味觉反应。