Spatial taste coding in mouse gustatory cortex

小鼠味觉皮层的空间味觉编码

基本信息

批准号：
10382393
负责人：
John D Boughter
金额：
$ 38.67万
依托单位：
UNIVERSITY OF TENNESSEE HEALTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10382393
关键词：
Amygdaloid structure Anatomy Anorexia Anterior Area Aversive Stimulus Behavior Behavior Control Brain Calcium Cells Code Conflict (Psychology)Contralateral Coronary Arteriosclerosis Data Analyses Diabetes Mellitus Dorsal Eating Electrophysiology (science)Endoscopes Extinction (Psychology)Goals Health Human Hypertension Image Imaging Techniques Implant Interneurons Ipsilateral Knowledge Label Learning Light Location Mammals Maps Measures Multivariate Analysis Mus Neurons Obesity Operative Surgical Procedures Palate Phenotype Play Population Process Pyramidal Cells Rattus Research Resolution Rest Role Stimulus Taste Perception Taste aversion Testing Time Transgenic Mice Variant Work awake base brain pathway cell type classical conditioning experimental study hedonic hippocampal pyramidal neuron imaging study in vivo in vivo imaging insight learned behavior learning extinction response taste stimuli two-photon

项目摘要

Project Summary Ingestive decisions play a key role in a number of human conditions including obesity, diabetes, anorexia, hypertension, and coronary artery disease. One of the most important factors regulating these decisions is the sense of taste. The gustatory cortex (GC) in mammals has been shown to be involved in taste learning and behavior, and electrophysiological and imaging studies suggest taste quality information is encoded in the activity of both specific and broadly responsive taste-activated neurons. However, it is not clear how taste quality is spatially organized across this brain area. We will use two-photon (2P) imaging to systematically map taste quality across the breadth of GC, evaluating the hypothesis that it has an overlapping organization, with likely overrepresentation of particular tastes at the anterior and posterior extremes. We will also combine 2P imaging with specific identification of cell types, accomplished by either Cre-dependent expression or retrograde labeling. Finally we will evaluate taste responses in GC following taste learning. Collectively, these experiments will shed light on how tastes are organized in this key brain area at a cellular level, and how they are modified with behavior. Aim 1 will systematically map taste responses along the anterior-posterior axis, and also with respect to dorsal-ventral location and depth. Aim 2 will compare taste responses in Thy1- expressing pyramidal cells and GAD-expressing interneurons to the greater population of all labeled cells in GC. Taste responses will also be examined in cells that project to contralateral cortex, or to amygdala. Aim 3 will investigate taste responses in GC neurons following taste aversion learning and extinction.

项目摘要摄取决定在许多人类状况中起着关键作用，包括肥胖，糖尿病，厌食症，高血压和冠状动脉疾病。调节这些决定的最重要的因素之一是品味感。哺乳动物中的糖皮质（GC）已显示与味觉学习有关和行为以及电生理学和成像研究表明，味道质量信息已编码特定和广泛响应味觉激活的神经元的活性。但是，尚不清楚如何品味质量在该大脑区域遍布空间。我们将使用两个光（2p）成像进行系统地成像在GC广度上的地图品味质量，评估了它具有重叠组织的假设，在前后极端的特定口味可能过多。我们还将结合 2P成像具有特定的细胞类型鉴定，通过CRE依赖性表达或逆行标签。最后，我们将在味觉学习后评估GC中的口味反应。总的来说，这些实验将阐明在细胞水平的关键大脑区域中如何组织口味，以及它们如何通过行为修改。 AIM 1将沿前后轴系统地绘制味觉响应，以及关于背腹侧位置和深度。 AIM 2将比较Thy1- 向所有标记的细胞中较大种群表达锥体细胞和表达GAD的中间神经元 GC。还将在预测对侧皮层或杏仁核的细胞中检查味道反应。目标3 在口味厌恶学习和灭绝之后，将研究GC神经元中的味觉反应。