Design and Validation of the Utah Multisite Electrode Array (UMEA)

犹他多点电极阵列 (UMEA) 的设计和验证

基本信息

批准号：
8997542
负责人：
Rajmohan Bhandari
金额：
$ 41.24万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2018-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8997542
关键词：
Action Potentials Active Sites Adhesions Animals Architecture Area Biological Neural Networks Brain Brain Mapping Cereport Characteristics Chronic Clinical Clinical Trials Communities Complex Computers Data Deposition Detection Development Devices Electrodes Engineering Extravasation FDA approved Felis catus Future Goals Human In Vitro Individual Ions Location Maps Measurement Measures Metals Modeling Neurons Neurosciences Noise Pattern Performance Physiologic pulse Physiological Platinum Prevalence Procedures Process Property Publishing Records Reproducibility Research Research Personnel Resolution Scanning Electron Microscopy Shapes Signal Transduction Site Source Spectrum Analysis Speed Structure Surface Techniques Technology Testing Time Tissues Utah Validation Writing base density design electric impedance flexibility improved in vitro testing in vivo innovation millimeter nanometer neuroregulation novel public health relevance relating to nervous system success tool

项目摘要

DESCRIPTION (provided by applicant): The technological advancements in neural engineering have provided an increasingly more powerful toolset of designs, materials, components and integrated devices for establishing high-fidelity chronic neural interfaces. A primary requirement of these neural interfaces for majority of the neuroscience studies is the ability to simultaneously record and/or stimulate from a large neuronal aggregates for specific periods of time. The future progress in neuroscience to a large extent relies on the ability of these neural interfaces to allow simultaneous observation and experimental access of complex neural networks and properties of cooperating neurons. Two critical solutions for achieving this goal are placing a large number of electrode sites in a small amount of tissue at the sub-millimeter range without significant tissue damage and efficient isolation of action potentials emanating from individual neurons. These solutions have remained largely unexploited mainly because of technological challenges, inherent limitations in design tolerances, and non-standard manufacturing techniques used in existing neural devices. Today, most of the success achieved in understating the physiological function of the brain is based on the sequential analysis of single-site recordings. And one neural interface, which has been successful in achieving this is the Utah electrode array (UEA), the only FDA approved commercialized device that has been extensively used in human clinical trials. Although the reasons are debatable as to its prevalence, the UEA records a rich feature set of neuronal information by distributing its electrodes at regular spacing over a large region across the cortical surface. However, this also implies that the data on any given electrode may be the only source of such information and, hence, it is not a robust source of information. As a result there has been a long-desire in the neuroscience community for having the ability to have multiple active sites distributed along each UEA electrode shanks, which could give it the robustness in feature extraction but not at the expense of losing its ability to record across the a wide region of cortical surface. We have developed a novel focused ion beam (FIB) technology that allows fabricating multiple sites on the shafts of the UEA. The FIB technology allows one to literally "write" with platinum on the shaft of the UEA with precise control and <1 um resolution. As the underlying structure of the proposed Utah Multisite electrode array (UMEA) is a UEA, our proposed innovation does not lose the value of the standard UEA but does gain the advantage of robustness in detection of neuronal sources. Furthermore, the flexibility in patterning multiple sites on each shank readily allows the creation of tetrode and laminar configurations of multisites on the shaft of the UEA so that the device can be tailored to the task. Also the electrode sites can be realized from a variety of materials, can have a range of surface areas, and can be placed anywhere along the shanks at any spacing. The objective of this research is to design, investigate and validate different configurations of high density (56 electrodes/mm2) UMEA (specific aim-I). We will perform in-vitro testing (specific aim-II) and in-vivo validation and comparison of recording performance of different configuration of the UMEA (specific aim-III). The presented innovation and objectives in this proposal will open a whole spectrum of new possibilities for the neuroscience researcher. It is envisioned that the UMEA will be a better tool for understanding neuronal activity by providing recordings sites in a three dimensional region of cortex. The ease and flexibility of incorporating any multisite design of on the UEA shanks makes the presented approach simple and yet efficient. As a result, the proposed study will be a shortest path towards product validation (device and animal) and the clinical implementation of a new electrode technology (UMEA).

描述（由申请人提供）：神经工程的技术进步提供了越来越强大的设计，材料，组件和集成设备的工具集，用于建立高保真慢性神经接口。这些神经界面对大多数神经科学研究的主要要求是能够在特定时间段内从大型神经元聚集体中同时记录和/或刺激。神经科学的未来进步在很大程度上取决于这些神经界面的能力，可以同时观察复杂的神经网络和合作神经元的特性。实现这一目标的两种关键解决方案是将大量的电极位点放在少量的组织中，在亚毫米范围内，没有明显的组织损伤和有效隔离从单个神经元发出的动作电位。这些解决方案主要是由于技术挑战，设计公差的固有局限性以及现有神经设备中使用的非标准制造技术的主要原因。如今，在低估大脑的生理功能方面取得的大部分成功都是基于单位记录的顺序分析。一个神经界面已成功实现这一目标是犹他州电极阵列（UEA），这是FDA批准的商业化设备，已在人类临床试验中广泛使用。尽管原因是其患病率是有争议的，但UEA通过在跨皮质表面的一个大区域上分配其电极来记录一组丰富的神经元信息集。但是，这也意味着任何给定电极上的数据可能是此类信息的唯一来源，因此，它不是强大的信息来源。结果，神经科学界已经有一个长期发作的能力，该能力具有沿每个UEA电极柄分布多个活性位点的能力，这可以使其具有特征提取的稳健性，但不能以失去其在整个皮质表面上记录的能力。我们已经开发了一种新型的聚焦离子束（FIB）技术，该技术允许在UEA轴上制造多个位点。 FIB技术使人们可以通过精确控制和<1 UM分辨率在UEA的轴上用白金“写”。由于拟议的犹他州多站点电极阵列（UMEA）的基础结构是UEA，因此我们提出的创新不会失去标准UEA的值，而是获得了鲁棒性在检测神经元来源方面的优势。此外，在每个小腿上对多个位点进行构图的灵活性易于在UEA轴上创建多站点的四极管和层状配置，从而可以为任务量身定制设备。此外，电极位点可以从多种材料中实现，可以具有一系列的表面区域，并且可以在任何间距沿柄的任何地方放置。这项研究的目的是设计，调查和验证高密度（56电极/mm2）UMEA（特定AIM-I）的不同构型。我们将执行视频内测试（特定的AIM-II）和体内验证以及UMEA不同配置（特定AIM-III）的记录性能的比较。本提案中提出的创新和目标将为神经科学研究人员开辟一系列新的可能性。可以预见，UMEA将通过在皮质的三维区域提供记录位点来理解神经元活动的更好工具。在UEA柄上合并任何多站点设计的便捷性和灵活性使提出的方法简单而有效。结果，拟议的研究将是通往产品验证（设备和动物）的最短途径，以及新电极技术（UMEA）的临床实施。