Lateral Hypothalamic Circuits for Feeding and Reinforcement

用于喂养和强化的外侧下丘脑回路

基本信息

批准号：
10588126
负责人：
Garret D Stuber
金额：
$ 45.81万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-05-01 至 2026-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588126
关键词：
Anatomy Animal Feed Animals Appetitive Behavior Architecture Area Behavioral Brain Brain region Calcium Cell physiology Cells Code Communities Consummatory Behavior Consumption Data Data Set Desire for food Disease Dynorphins Electric Stimulation Elements Fasting Food Foundations Funding Future Gene Expression Gene Expression Profile Gene Expression Profiling Genes Genetic Genetic Transcription Glutamates Goals Habenula Head Hour Hypothalamic structure Image Individual Lateral Lateral Hypothalamic Area Link Mental disorders Modification Molecular Molecular Profiling Monitor Motivation Mus Neuroanatomy Neurons Neuropeptides Neurotensin Organism Output Pathway interactions Pharmacology Study Play Population Process Psychological reinforcement Research Resources Response to stimulus physiology Rewards Rodent Role Satiation Seminal Shapes Signal Transduction Site Somatostatin Stimulus Sucrose Supporting Cell Surveys Testing Thalamic structure Therapeutic Intervention Ventral Tegmental Area Ventricular Viral Work addiction brain circuitry cell type data resource differential expression drug of abuse experimental study feeding insight interdisciplinary approach motivated behavior neural circuit neuropsychiatric disorder novel optogenetics parabrachial nucleus reward processing single-cell RNA sequencing temporal measurement tool two-photon

项目摘要

Abstract: Motivated behaviors such as feeding and reward-seeking are critical for an organism's survival. These processes require distributed neuronal networks and supporting cell types in multiple brain regions to be tightly regulated and tuned in order to orchestrate behavioral output. The lateral hypothalamic area (LHA) has long been identified as a critical neuroanatomical substrate for motivated behavior. Despite decades of research, the molecular identity of defined LHA cell types remains poorly understood. Additionally, while LHA neurons have been previously shown to encode appetitive and consummatory behaviors via distinct cellular ensembles, it remains unknown how distinct LHA output neurons contribute to these processes. Here we propose to continue to study the neural circuits of the LHA, and to identify how distinct cell types contribute to feeding and reinforcement. Using an interdisciplinary approach to leverage cutting edge tools such as single cell transcriptional profiling, two photon calcium imaging, and viral we aim to undercover key circuit elements, and novel circuit specific gene expression patterns that can be leveraged for future therapeutic interventions for addiction and other neuropsychiatric disorders.

抽象的：进食和寻求奖励等动机行为对于有机体的生存至关重要。这些过程需要分布式神经元网络和多个大脑区域中的支持细胞类型紧密结合进行调节和调整，以协调行为输出。下丘脑外侧区（LHA）长期被认为是动机行为的关键神经解剖学基础。尽管经过数十年的研究，对特定 LHA 细胞类型的分子特性仍知之甚少。此外，虽然 LHA 神经元之前已被证明可以通过不同的细胞整体编码食欲和完成行为，目前尚不清楚不同的 LHA 输出神经元如何促进这些过程。在此我们建议继续研究 LHA 的神经回路，并确定不同的细胞类型如何有助于进食和加强。使用跨学科方法来利用单细胞等尖端工具转录分析、双光子钙成像和病毒，我们的目标是发现关键的电路元件，以及新颖的电路特异性基因表达模式，可用于未来的治疗干预成瘾和其他神经精神疾病。