Molecular Mechanisms

分子机制

• 批准号: 10569515
• 负责人: J Michael Salbaum
• 金额: $ 52.92万
• 依托单位: LSU PENNINGTON BIOMEDICAL RESEARCH CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10569515
• 关键词: 3-Dimensional; Affect; Applications Grants; Biological; Biomedical Research; Biomedical Technology; Cells; Centers of Research Excellence; Complex; Computer software; Data; Data Collection; Data Set; Disease; Ensure; Environment; Equipment; Etiology; Evaluation; Experimental Designs; Expression Profiling; Funding; Future; Gene Expression; Generations; Genomic approach; Genomics; Goals; Histology; Human Resources; Individual; Informatics; Knowledge; Laboratories; Leadership; Light; Maintenance; Measurement; Mentors; Metabolic; Methods; Microscopy; Modernization; Molecular; Outcome; Ownership; Pathogenicity; Principal Investigator; Productivity; RNA; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Running; Science; Secure; Solid; Systems Biology; Technical Expertise; Technology; Three-Dimensional Imaging; Tissues; United States National Institutes of Health; Visualization; bioimaging; career; cost; design; experimental study; functional genomics; gene product; imaging approach; imaging modality; insight; instrumentation; new technology; protocol development; single-cell RNA sequencing; skills; sound; success

Project Summary: Modern biomedical research to uncover the metabolic basis of disease is increasingly dependent on the application of complex technology. In many cases, the costs associated with the acquisition and maintenance of necessary equipment, in addition to the technical expertise needed to run these technologies, typically exceed the capabilities of individual laboratories - particularly those of investigators at the start of their careers. Hence, the overarching goal for the COBRE Molecular Mechanisms Core is to provide investigators with such technologies in the realm of functional genomics and molecular bioimaging, and thus help them to apply state of the art approaches in their research projects. The Core seeks to achieve this goal in two Specific Aims: SPECIFIC AIM 1: To provide COBRE investigators with mentoring support concerning molecular bioimaging and functional genomics technology. Application of modern biomedical technology per se does not lead to meaningful or impactful scientific progress; it requires a clear understanding of the power of specific technologies for science to emerge from data. Therefore, the Core will serve as an intellectual and advisory resource for COBRE investigators when it comes to bioimaging and functional genomics experiments, and provide mentoring so that technological approaches match up with the scientific goal of each project. Hence, the goal for this Aim is to ensure that COBRE investigators, rather than viewing technology as a perhaps unsurmountable obstacle, can in fact embrace these new technologies with confidence to further their science. SPECIFIC AIM 2: To provide COBRE investigators with direct technological support for their experiments that use molecular bioimaging and functional genomics approaches. To understand the molecular etiology that forms the pathogenic framework for disease, technologies that capture gene expression changes are of primary necessity for conducting mechanistic research, and the key technologies for the Core are three-dimensional imaging of gene expression in whole-mount tissues, and assessment of gene expression using single-cell RNA sequencing. The Core will provide COBRE investigators with turn-key access to these technologies in order to ensure that they do not get needlessly bogged down in the hands-on technical minutiae, but can fully concentrate on the science of their research projects, and on their overall productivity. Furthermore, the Core, by relying on its NIH proposal review expertise, and by ensuring that experiments meet NIH rigor and reproducibility standards and thus contribute to a strong premise, can provide the mentoring environment needed for COBRE investigators to generate competitive NIH grant proposals.

项目概要： 现代生物医学研究揭示疾病的代谢基础越来越依赖于 复杂技术的应用。在许多情况下，与采购和维护相关的成本 除了运行这些技术所需的技术专业知识之外，必要的设备通常会超过 各个实验室的能力——特别是研究人员在职业生涯初期的能力。因此， COBRE 分子机制核心的总体目标是为研究人员提供此类 功能基因组学和分子生物成像领域的技术，从而帮助他们应用状态 他们的研究项目中的艺术方法。核心力求通过两个具体目标来实现这一目标： 具体目标 1：为 COBRE 研究人员提供有关分子生物成像和 功能基因组学技术。现代生物医学技术的应用本身并不导致 有意义或有影响力的科学进步；它需要清楚地了解特定技术的力量 让科学从数据中显现出来。因此，核心将作为知识和咨询资源 COBRE 研究人员在生物成像和功能基因组学实验方面提供指导 使技术方法与每个项目的科学目标相匹配。因此，这个目标的目标 是为了确保 COBRE 调查人员不会将技术视为可能无法克服的障碍， 事实上，我们可以充满信心地接受这些新技术，以进一步发展他们的科学。 具体目标 2：为 COBRE 研究人员的实验提供直接技术支持 使用分子生物成像和功能基因组学方法。了解形成的分子病因学 在疾病的致病框架中，捕获基因表达变化的技术是首要的 进行机理研究的必要性，核心关键技术是三维的 全组织基因表达成像，以及使用单细胞 RNA 评估基因表达 测序。 Core 将为 COBRE 调查人员提供对这些技术的交钥匙访问权限，以便 确保他们不会不必要地陷入实际操作的技术细节中，而是能够完全集中精力 他们的研究项目的科学性以及他们的整体生产力。 此外，核心依靠其 NIH 提案审查专业知识，并确保实验符合 NIH 的严格性和可重复性标准因而有助于提供强有力的前提，可以提供指导 COBRE 研究人员生成有竞争力的 NIH 资助提案所需的环境。