Tagging of Laboratory Mice with Microtransponders

用微转发器标记实验室小鼠

• 批准号: 8136100
• 负责人: WLODEK MANDECKI
• 金额: $ 66.65万
• 依托单位: PHARMASEQ, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8136100
• 关键词: Acoustics; Address; Adhesives; Adoption; Aging; Algorithms; Amplifiers; Animal Welfare; Animals; Architecture; Back; Basic Science; Behavior assessment; Biocompatible; Biomedical Research; Boxing; Breeding; Characteristics; Collaborations; Computer software; Computers; Data; Databases; Dentistry; Detection; Development; Dimensions; Disease; Elements; Embryo; Evaluation; Excision; Feasibility Studies; Feedback; Frequencies; Fuzzy Logic; Genetic Research; Genetic Variation; Goals; Health; Housing; Human; Immunocompetent; Implant; Inherited; Investigation; Label; Laboratories; Laboratory Research; Laboratory mice; Lasers; Laws; Lead; Light; Liquid substance; Magnetism; Mammalian Genetics; Mechanics; Medicine; Metals; Modeling; Mouse Strains; Mus; Names; Needles; Neurosciences; New Jersey; Nitrogen; Noise; Pathologic; Pattern; Pennsylvania; Pharmacologic Substance; Phase; Phenotype; Play; Procedures; Process; Radio; Reader; Reading; Recording of previous events; Records; Research; Rivers; Robot; Role; Scheme; Schools; Semiconductors; Shapes; Signal Transduction; Silicon; Skin; Sorting - Cell Movement; Staging; Surface; System; Tail; Techniques; Testing; Thick; Time; Transistors; United States National Institutes of Health; Universities; Veterinary Medicine; Width; Work; analog; animal breeding; attenuation; base; biomaterial compatibility; computerized data processing; cost; design; detector; digital; immunosuppressed; improved; new technology; oncology; operation; parylene; prevent; programs; public health relevance; sensor; software systems

DESCRIPTION (provided by applicant): The purpose of the present project is to develop a new type of RFID tagging system for tracking mice. It is based on the PharmaSeq laser light-activated microtransponder, also known as "p-Chip". Its major advantages are its small size (500 5m x 500 5m x 100 5m), monolithic (one part) design, inertness (silicon-based), ease of use and very low cost. No anesthetization of the mice is required. The system consists of the tag itself, the tag reader, and an injector for p-Chips. The p-Chips are rapidly implanted in a mouse's tail just under the skin. To identify the animal, the operator simply brings a reader to within the immediate proximity of the implanted p-Chip and the ID is acquired automatically and transmitted to the a computer. The software further associates the ID of a particular mouse with data related to its role in experimental procedures and/or breeding history. The main goals of the project are to: (1) make several improvements to the injector regarding the ease of use, and fabricate a larger number of units; (2) conduct animal studies including biocompatibility in immunocompetent as well as immunosuppressed strains of mice; (3) modify the existing p-Chip/ID reader system, including the digital board and software; and (4) redesign of p-Chip/receiver system to work at higher frequencies (315 MHz). The system will be thoroughly tested in-house and in collaboration with outside laboratories. Tagging with p-Chips will improve the reliability of tracking of experimental subjects by preventing costly errors including rework due do unreadable markings and lost labels. PUBLIC HEALTH RELEVANCE: Laboratory mice play a powerful role in basic research and a key role in mammalian genetic and biomedical research. They are universally accepted as the primary model for analyzing and understanding inherited human disorders. The new PharmaSeq system has the potential to greatly increase the use of RFID tags in laboratory mice, or even to lead to the general adoption of this new technology as a universal tagging system that can track animals from breeding through their entire useful lifetime.

描述（由申请人提供）：本项目的目的是开发一种用于跟踪小鼠的新型RFID标记系统。它基于Pharmaseq激光激活的微转带，也称为“ P-Chip”。它的主要优点是其尺寸小（500 5m x 500 5m x 100 5m），整体设计（一部分）设计，惰性（基于硅），易用性和非常低的成本。不需要小鼠麻醉。该系统由标签本身，标签读取器和P-Chips的喷油器组成。 P-CHIP迅速植入了皮肤下方的鼠标尾巴。为了识别动物，操作员只需将读者带到植入的P-Chip的直接距离之内，并自动获取ID并将其传输到计算机。该软件将特定鼠标的ID与与其在实验程序和/或育种历史记录中的作用相关的数据相关联。该项目的主要目标是：（1）在易用性方面对注射器进行了一些改进，并制造了更多的单位； （2）进行动物研究，包括在免疫能力和免疫抑制小鼠菌株中生物相容性； （3）修改现有的P-CHIP/ID读取器系统，包括数字板和软件； （4）重新设计P-Chip/接收器系统以更高的频率（315 MHz）工作。该系统将在内部进行彻底测试，并与外部实验室合作。使用P-CHIP进行标记将通过防止昂贵的错误（包括返工应得的不可读的标记和丢失的标签）来提高跟踪实验对象的可靠性。 公共卫生相关性：实验室小鼠在基础研究中起着强大的作用，并且在哺乳动物遗传和生物医学研究中起着关键作用。它们被普遍接受为分析和理解遗传性人类疾病的主要模型。新的PharmaseQ系统有可能大大增加实验室小鼠中RFID标签的使用，甚至可以使这项新技术一般采用作为通用标记系统，可以追踪动物从繁殖到整个有用的寿命。

项目成果

A system for implanting laboratory mice with light-activated microtransponders.

一种为实验室小鼠植入光激活微应答器的系统。

• 发表时间: 2010
• 期刊: Journal of the American Association for Laboratory Animal Science : JAALAS
• 作者: Gruda,MaryannC;Pinto,Amanda;Craelius,Aaron;Davidowitz,Hanan;Kopacka,WesleyM;Li,Ji;Qian,Jay;Rodriguez,Efrain;Kuspiel,Edward;Mandecki,Wlodek
• 通讯作者: Mandecki,Wlodek